RRC ID 22012
Author Rahn T, Leippe M, Roeder T, Fedders H.
Title EGFR signaling in the brain is necessary for olfactory learning in Drosophila larvae.
Journal Learn Mem
Abstract Signaling via the epidermal growth factor receptor (EGFR) pathway has emerged as one of the key mechanisms in the development of the central nervous system in Drosophila melanogaster. By contrast, little is known about the functions of EGFR signaling in the differentiated larval brain. Here, promoter-reporter lines of EGFR and its most prominent activating ligands, Spitz, Keren, and Vein, were used to identify the brain structures relevant for the EGFR pathway. Unexpectedly, promoter activity of all these pathway components was found in the mushroom bodies, which are known to be a higher brain center required for olfactory learning. We investigated the role of the EGFR pathway in this process by using different mutant larvae with reduced pan-neuronal EGFR signaling and those with reduced EGFR signaling in mushroom bodies only. Expression of a dominant-negative form of EGFR as well as silencing of the ligands via RNA interference was applied and resulted in significantly impaired olfactory learning performances. General defects in the ability to taste or smell as well as impaired EGFR signaling during embryonic development could be excluded as major reasons for this learning phenotype. In addition, targeted expression of a constitutively active form of the ligand Spitz also led to a significantly reduced learning ability. Thus, very low levels as well as very high levels of EGFR signaling are deleterious for olfactory learning and memory formation. We hypothesize that EGFR signaling in a certain range maintains a homeostatic situation in the mushroom bodies that is necessary for proper learning and memory.
Volume 20(4)
Pages 194-200
Published 2013-3-19
DOI 10.1101/lm.029934.112
PII 20/4/194
PMID 23512935
MeSH Animals Animals, Genetically Modified Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* ErbB Receptors / genetics ErbB Receptors / metabolism* Food Preferences / physiology Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Larva Learning / physiology* Mushroom Bodies / metabolism* Mushroom Bodies / physiology Odorants Olfactory Pathways / physiology* RNA Interference / physiology Receptors, Invertebrate Peptide / genetics Receptors, Invertebrate Peptide / metabolism* Signal Transduction / genetics Signal Transduction / physiology* Transcription Factors / genetics Transcription Factors / metabolism
IF 2.359
Times Cited 13
Drosophila DGRC#108189 and ...