Reference - Detail
| RRC ID | 2221 |
|---|---|
| Author | Iwaki T, Morita T, Tanaka N, Giga-Hama Y, Takegawa K. |
| Title | Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Schizosaccharomyces pombe has four alpha-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Delta was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Delta, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Delta had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting. |
| Volume | 71(2) |
| Pages | 623-6 |
| Published | 2007-2-1 |
| DOI | 10.1271/bbb.60609 |
| PII | JST.JSTAGE/bbb/60609 |
| PMID | 17284820 |
| MeSH | Cathepsin A / metabolism Fluorescence Glycosylphosphatidylinositols / genetics Glycosylphosphatidylinositols / physiology* Membrane Proteins / genetics Membrane Proteins / physiology* Microscopy, Fluorescence Mutation / physiology Plasmids / genetics Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Vacuoles / genetics Vacuoles / metabolism* alpha-Amylases / genetics alpha-Amylases / metabolism alpha-Amylases / physiology* |
| IF | 1.516 |
| Times Cited | 2 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Yeast | pTN197 |