論文 - 詳細
| RRC ID | 2225 |
|---|---|
| 著者 | Kawashima SA, Tsukahara T, Langegger M, Hauf S, Kitajima TS, Watanabe Y. |
| タイトル | Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres. |
| ジャーナル | Genes Dev |
| Abstract |
Fission yeast shugoshin Sgo1 is meiosis specific and cooperates with protein phosphatase 2A to protect centromeric cohesin at meiosis I. The other shugoshin-like protein Sgo2, which requires the heterochromatin protein Swi6/HP1 for full viability, plays a crucial role for proper chromosome segregation at both mitosis and meiosis; however, the underlying mechanisms are totally elusive. We here demonstrate that, unlike Sgo1, Sgo2 is dispensable for centromeric protection of cohesin. Instead, Sgo2 interacts with Bir1/Survivin and promotes Aurora kinase complex localization to the pericentromeric region, to correct erroneous attachment of kinetochores and thereby enable tension-generating attachment. Forced localization of Bir1 to centromeres partly restored the defects of sgo2Delta. This newly identified interaction of shugoshin with Survivin is conserved between mitosis and meiosis and presumably across eukaryotes. We propose that ensuring bipolar attachment of kinetochores is the primary role of shugoshin and the role of cohesion protection might have codeveloped to facilitate this process. |
| 巻・号 | 21(4) |
| ページ | 420-35 |
| 公開日 | 2007-2-15 |
| DOI | 10.1101/gad.1497307 |
| PII | 21/4/420 |
| PMID | 17322402 |
| PMC | PMC1804331 |
| MeSH | Aurora Kinases Centromere / enzymology* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosome Pairing* Chromosome Segregation Fungal Proteins / analysis Fungal Proteins / metabolism Gene Deletion Kinetochores / metabolism* Mitosis Protein Serine-Threonine Kinases / analysis Protein Serine-Threonine Kinases / antagonists & inhibitors Protein Serine-Threonine Kinases / metabolism* RNA Interference Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / analysis Schizosaccharomyces pombe Proteins / antagonists & inhibitors Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 9.527 |
| 引用数 | 140 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 酵母 | FY13725(PY615) FY13718(PY532) |