RRC ID 2235
Author Ding DQ, Sakurai N, Katou Y, Itoh T, Shirahige K, Haraguchi T, Hiraoka Y.
Title Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast.
Journal J. Cell Biol.
Abstract The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. Conversely, loss of the cohesin-associated protein Pds5 resulted in hypercompaction. Although this hypercompaction requires Rec8, binding of Rec8 to chromatin was reduced in the absence of Pds5, indicating that Pds5 promotes chromosome association of Rec8. To explain these observations, we propose that meiotic prophase chromosomes are organized as chromatin loops emanating from a Rec8-containing axis: the absence of Rec8 disrupts the axis, resulting in disorganized chromosomes, whereas reduced Rec8 loading results in a longitudinally compacted axis with fewer attachment points and longer chromatin loops.
Volume 174(4)
Pages 499-508
Published 2006-8-14
DOI 10.1083/jcb.200605074
PII jcb.200605074
PMID 16893973
PMC PMC2064256
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Polarity / physiology Chromosomes / genetics Chromosomes / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Meiosis / physiology* Mutation / genetics Phosphoproteins / genetics Phosphoproteins / metabolism* Prophase / physiology* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sister Chromatid Exchange / physiology
IF 8.891
Times Cited 46
Yeast FY15623(YY231-5C) FY15624(YY231-6C) FY15615(AY278-7D) FY15640(MK101) FY13716(PY468)