RRC ID 2239
Author Morita T, Tanaka N, Hosomi A, Giga-Hama Y, Takegawa K.
Title An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe.
Journal Biosci. Biotechnol. Biochem.
Abstract Glycosylphosphatidylinositol (GPI)-anchored proteins are essential for normal cellular morphogenesis and have an additional role in mediating cross-linking of glycoproteins to cell wall glucan in yeast cells. Although many GPI-anchored proteins have been characterized in Saccharomyces cerevisiae, none have been reported for well-characterized GPI-anchored proteins in Schizosaccharomyces pombe to date. Among the putative GPI-anchored proteins in S. pombe, four alpha-amylase homologs (Aah1p-Aah4p) have putative signal sequences and C-terminal GPI anchor addition signals. Disruption of aah3(+) resulted in a morphological defect and hypersensitivity to cell wall-degrading enzymes. Biochemical analysis showed that Aah3p is an N-glycosylated, GPI-anchored membrane protein localized in the membrane and cell wall fractions. Conjugation and sporulation were not affected by the aah3(+) deletion, but the ascal wall of aah3Delta cells was easily lysed by hydrolases. Expression of aah3 alleles in which the conserved aspartic acid and glutamic acid residues required for hydrolase activity were replaced with alanine residues failed to rescue the morphological and ascal wall defects of aah3Delta cells. Taken together, these results indicate that Aah3p is a GPI-anchored protein and is required for cell and ascal wall integrity in S. pombe.
Volume 70(6)
Pages 1454-63
Published 2006-6
PII JST.JSTAGE/bbb/50693
PMID 16751704
MeSH Actins / metabolism Amino Acid Sequence Cell Shape Cell Wall / metabolism* Conserved Sequence Glycosylation Glycosylphosphatidylinositols / metabolism* Hydrolysis Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Mutation / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Schizosaccharomyces pombe Proteins / physiology Sequence Alignment alpha-Amylases / classification alpha-Amylases / genetics alpha-Amylases / metabolism* alpha-Amylases / physiology
IF 1.297
Times Cited 12
Yeast KJ100-7B