論文 - 詳細
| RRC ID | 2243 |
|---|---|
| 著者 | Harigaya Y, Tanaka H, Yamanaka S, Tanaka K, Watanabe Y, Tsutsumi C, Chikashige Y, Hiraoka Y, Yamashita A, Yamamoto M. |
| タイトル | Selective elimination of messenger RNA prevents an incidence of untimely meiosis. |
| ジャーナル | Nature |
| Abstract |
Much remains unknown about the molecular regulation of meiosis. Here we show that meiosis-specific transcripts are selectively removed if expressed during vegetative growth in fission yeast. These messenger RNAs contain a cis-acting region--which we call the DSR--that confers this removal via binding to a YTH-family protein Mmi1. Loss of Mmi1 function severely impairs cell growth owing to the untimely expression of meiotic transcripts. Microarray analysis reveals that at least a dozen such meiosis-specific transcripts are eliminated by the DSR-Mmi1 system. Mmi1 remains in the form of multiple nuclear foci during vegetative growth. At meiotic prophase these foci precipitate to a single focus, which coincides with the dot formed by the master meiosis-regulator Mei2. A meiotic arrest due to the loss of the Mei2 dot is released by a reduction in Mmi1 activity. We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts. |
| 巻・号 | 442(7098) |
| ページ | 45-50 |
| 公開日 | 2006-7-6 |
| DOI | 10.1038/nature04881 |
| PII | nature04881 |
| PMID | 16823445 |
| MeSH | Meiosis / genetics* Mitosis / genetics Prophase RNA, Messenger / genetics* RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Time Factors Transcription, Genetic / genetics mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism |
| IF | 42.779 |
| 引用数 | 199 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| リソース情報 | |
| 酵母 | FY13309(JZ464) FY13448(JX383) |