Reference - Detail
|Author||Harigaya Y, Tanaka H, Yamanaka S, Tanaka K, Watanabe Y, Tsutsumi C, Chikashige Y, Hiraoka Y, Yamashita A, Yamamoto M.|
|Title||Selective elimination of messenger RNA prevents an incidence of untimely meiosis.|
Much remains unknown about the molecular regulation of meiosis. Here we show that meiosis-specific transcripts are selectively removed if expressed during vegetative growth in fission yeast. These messenger RNAs contain a cis-acting region--which we call the DSR--that confers this removal via binding to a YTH-family protein Mmi1. Loss of Mmi1 function severely impairs cell growth owing to the untimely expression of meiotic transcripts. Microarray analysis reveals that at least a dozen such meiosis-specific transcripts are eliminated by the DSR-Mmi1 system. Mmi1 remains in the form of multiple nuclear foci during vegetative growth. At meiotic prophase these foci precipitate to a single focus, which coincides with the dot formed by the master meiosis-regulator Mei2. A meiotic arrest due to the loss of the Mei2 dot is released by a reduction in Mmi1 activity. We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts.
|MeSH||Meiosis / genetics* Mitosis / genetics Prophase RNA, Messenger / genetics* RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Time Factors Transcription, Genetic / genetics mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism|
|WOS Category||MULTIDISCIPLINARY SCIENCES|