RRC ID 2243
Author Harigaya Y, Tanaka H, Yamanaka S, Tanaka K, Watanabe Y, Tsutsumi C, Chikashige Y, Hiraoka Y, Yamashita A, Yamamoto M.
Title Selective elimination of messenger RNA prevents an incidence of untimely meiosis.
Journal Nature
Abstract Much remains unknown about the molecular regulation of meiosis. Here we show that meiosis-specific transcripts are selectively removed if expressed during vegetative growth in fission yeast. These messenger RNAs contain a cis-acting region--which we call the DSR--that confers this removal via binding to a YTH-family protein Mmi1. Loss of Mmi1 function severely impairs cell growth owing to the untimely expression of meiotic transcripts. Microarray analysis reveals that at least a dozen such meiosis-specific transcripts are eliminated by the DSR-Mmi1 system. Mmi1 remains in the form of multiple nuclear foci during vegetative growth. At meiotic prophase these foci precipitate to a single focus, which coincides with the dot formed by the master meiosis-regulator Mei2. A meiotic arrest due to the loss of the Mei2 dot is released by a reduction in Mmi1 activity. We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts.
Volume 442(7098)
Pages 45-50
Published 2006-7-6
DOI 10.1038/nature04881
PII nature04881
PMID 16823445
MeSH Meiosis / genetics* Mitosis / genetics Prophase RNA, Messenger / genetics* RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Time Factors Transcription, Genetic / genetics mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism
IF 43.07
Times Cited 148
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
Yeast FY13309(JZ464) FY13448(JX383)