RRC ID 22706
Author Perkins AD, Ellis SJ, Asghari P, Shamsian A, Moore ED, Tanentzapf G.
Title Integrin-mediated adhesion maintains sarcomeric integrity.
Journal Dev Biol
Abstract Integrin-mediated adhesion to the ECM is essential for normal development of animal tissues. During muscle development, integrins provide the structural stability required to construct such a highly tensile, force generating tissue. Mutations that disrupt integrin-mediated adhesion in skeletal muscles give rise to a myopathy in humans and mice. To determine if this is due to defects in formation or defects in maintenance of muscle tissue, we used an inducible, targeted RNAi based approach to disrupt integrin-mediated adhesion in fully formed adult fly muscles. A decrease in integrin-mediated adhesion in adult muscles led to a progressive loss of muscle function due to a failure to maintain normal sarcomeric cytoarchitecture. This defect was due to a gradual, age dependent disorganization of the sarcomeric actin, Z-line, and M-line. Electron microscopic analysis showed that reduction in integrin-mediated adhesion resulted in detachment of actin filaments from the Z-lines, separation of the Z-lines from the membrane, and eventually to disintegration of the Z-lines. Our results show that integrin-mediated adhesion is essential for maintaining sarcomeric integrity and illustrate that the seemingly stable adhesive contacts underlying sarcomeric architecture are inherently dynamic.
Volume 338(1)
Pages 15-27
Published 2010-2-1
DOI 10.1016/j.ydbio.2009.10.034
PII S0012-1606(09)01293-7
PMID 19879257
MeSH Actinin / metabolism Actins / metabolism Aging / metabolism Animals Cell Adhesion Cell Differentiation Drosophila melanogaster / cytology* Drosophila melanogaster / enzymology Drosophila melanogaster / growth & development Drosophila melanogaster / ultrastructure Flight, Animal Gene Knockdown Techniques Integrins / metabolism* Models, Biological Muscles / metabolism Muscles / pathology Organ Specificity Phenotype Sarcomeres / metabolism* Sarcomeres / pathology Sarcomeres / ultrastructure Talin / metabolism Time Factors
IF 2.936
Times Cited 34
Drosophila 6831R-1 6831R-2 1560R-1 1560R-2 9623R-1 9623R-2 7762R-1 7761R-1