論文 - 詳細
| RRC ID | 22786 |
|---|---|
| 著者 | Li Z, Cheng D, Mon H, Tatsuke T, Zhu L, Xu J, Lee JM, Xia Q, Kusakabe T. |
| タイトル | Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori. |
| ジャーナル | PLoS One |
| Abstract |
Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, Polycomb repressive complex 1 (PRC1), Polycomb repressive complex 2 (PRC2), and Pleiohomeotic repressive complex (PhoRC), to repress transcription of the target genes. Here, we identified Polycomb target genes in Bombyx mori with holocentric centromere using genome-wide expression screening based on the knockdown of BmSCE, BmESC, BmPHO, or BmSCM gene, which represent the distinct complexes. As a result, the expressions of 29 genes were up-regulated after knocking down 4 PcG genes. Particularly, there is a significant overlap between targets of BmPho (331 out of 524) and BmScm (331 out of 532), and among these, 190 genes function as regulator factors playing important roles in development. We also found that BmPho, as well as BmScm, can interact with other Polycomb components examined in this study. Further detailed analysis revealed that the C-terminus of BmPho containing zinc finger domain is involved in the interaction between BmPho and BmScm. Moreover, the zinc finger domain in BmPho contributes to its inhibitory function and ectopic overexpression of BmScm is able to promote transcriptional repression by Gal4-Pho fusions including BmScm-interacting domain. Loss of BmPho expression causes relocalization of BmScm into the cytoplasm. Collectively, we provide evidence of a functional link between BmPho and BmScm, and propose two Polycomb-related repression mechanisms requiring only BmPho associated with BmScm or a whole set of PcG complexes. |
| 巻・号 | 7(4) |
| ページ | e34330 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0034330 |
| PII | PONE-D-11-24165 |
| PMID | 22485166 |
| PMC | PMC3317521 |
| MeSH | Amino Acid Sequence Animals Bombyx / genetics* Cell Proliferation Cells, Cultured Gene Expression Profiling Gene Expression Regulation Gene Knockdown Techniques Genes, Reporter Genome, Insect* Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Luciferases / biosynthesis Luciferases / genetics Molecular Sequence Data Oligonucleotide Array Sequence Analysis Polycomb-Group Proteins Protein Binding Protein Interaction Domains and Motifs Protein Transport RNA Interference Repressor Proteins / genetics* Repressor Proteins / metabolism Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 2.74 |
| 引用数 | 12 |
| WOS 分野 | CELL BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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