論文 - 詳細
| RRC ID | 22868 |
|---|---|
| 著者 | Yin Y, Smolikove S. |
| タイトル | Impaired resection of meiotic double-strand breaks channels repair to nonhomologous end joining in Caenorhabditis elegans. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Repair of double-strand DNA breaks (DSBs) by the homologous recombination (HR) pathway results in crossovers (COs) required for a successful first meiotic division. Mre11 is one member of the MRX/N (Mre11, Rad50, and Xrs2/Nbs1) complex required for meiotic DSB formation and for resection in Saccharomyces cerevisiae. In Caenorhabditis elegans, evidence for the MRX/N role in DSB resection is limited. We report the first separation-of-function allele, mre-11(iow1) in C. elegans, which is specifically defective in meiotic DSB resection but not in formation. The mre-11(iow1) mutants displayed chromosomal fragmentation and aggregation in late prophase I. Recombination intermediates and crossover formation was greatly reduced in mre-11(iow1) mutants. Irradiation-induced DSBs during meiosis failed to be repaired from early to middle prophase I in mre-11(iow1) mutants. In the absence of a functional HR, our data suggest that some DSBs in mre-11(iow1) mutants are repaired by the nonhomologous end joining (NHEJ) pathway, as removing NHEJ partially suppressed the meiotic defects shown by mre-11(iow1). In the absence of NHEJ and a functional MRX/N, meiotic DSBs are channeled to EXO-1-dependent HR repair. Overall, our analysis supports a role for MRE-11 in the resection of DSBs in middle meiotic prophase I and in blocking NHEJ. |
| 巻・号 | 33(14) |
| ページ | 2732-47 |
| 公開日 | 2013-7-1 |
| DOI | 10.1128/MCB.00055-13 |
| PII | MCB.00055-13 |
| PMID | 23671188 |
| PMC | PMC3700128 |
| MeSH | Amino Acid Sequence Animals Apoptosis Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Conserved Sequence DNA Breaks, Double-Stranded* DNA Cleavage DNA End-Joining Repair* Exodeoxyribonucleases / metabolism Genes, Lethal Meiotic Prophase I* Molecular Sequence Data Point Mutation Rad51 Recombinase / metabolism Recombination, Genetic Recombinational DNA Repair |
| IF | 3.611 |
| 引用数 | 24 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1842 |