RRC ID 22868
著者 Yin Y, Smolikove S.
タイトル Impaired resection of meiotic double-strand breaks channels repair to nonhomologous end joining in Caenorhabditis elegans.
ジャーナル Mol Cell Biol
Abstract Repair of double-strand DNA breaks (DSBs) by the homologous recombination (HR) pathway results in crossovers (COs) required for a successful first meiotic division. Mre11 is one member of the MRX/N (Mre11, Rad50, and Xrs2/Nbs1) complex required for meiotic DSB formation and for resection in Saccharomyces cerevisiae. In Caenorhabditis elegans, evidence for the MRX/N role in DSB resection is limited. We report the first separation-of-function allele, mre-11(iow1) in C. elegans, which is specifically defective in meiotic DSB resection but not in formation. The mre-11(iow1) mutants displayed chromosomal fragmentation and aggregation in late prophase I. Recombination intermediates and crossover formation was greatly reduced in mre-11(iow1) mutants. Irradiation-induced DSBs during meiosis failed to be repaired from early to middle prophase I in mre-11(iow1) mutants. In the absence of a functional HR, our data suggest that some DSBs in mre-11(iow1) mutants are repaired by the nonhomologous end joining (NHEJ) pathway, as removing NHEJ partially suppressed the meiotic defects shown by mre-11(iow1). In the absence of NHEJ and a functional MRX/N, meiotic DSBs are channeled to EXO-1-dependent HR repair. Overall, our analysis supports a role for MRE-11 in the resection of DSBs in middle meiotic prophase I and in blocking NHEJ.
巻・号 33(14)
ページ 2732-47
公開日 2013-7-1
DOI 10.1128/MCB.00055-13
PII MCB.00055-13
PMID 23671188
PMC PMC3700128
MeSH Amino Acid Sequence Animals Apoptosis Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Conserved Sequence DNA Breaks, Double-Stranded* DNA Cleavage DNA End-Joining Repair* Exodeoxyribonucleases / metabolism Genes, Lethal Meiotic Prophase I* Molecular Sequence Data Point Mutation Rad51 Recombinase / metabolism Recombination, Genetic Recombinational DNA Repair
IF 3.611
引用数 24
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1842