RRC ID 2434
Author Hatano SY, Tada M, Kimura H, Yamaguchi S, Kono T, Nakano T, Suemori H, Nakatsuji N, Tada T.
Title Pluripotential competence of cells associated with Nanog activity.
Journal Mech. Dev.
Abstract Nanog is a novel pluripotential cell-specific gene that plays a crucial role in maintaining the undifferentiated state of early postimplantation embryos and embryonic stem (ES) cells. We have explored the expression pattern and function of Nanog and a Nanog-homologue, Nanog-ps1.Nanog-ps1 was mapped on Chromosome 7 and shown to be a pseudogene. Immunocytochemical analysis in vivo showed that the NANOG protein was absent in unfertilized oocytes, and was detected in cells of morula-stage embryos, the inner cell mass of blastocysts and the epiblast of E6.5 and E7.5 embryos, but not in primordial germ cells of early postimplantation embryos. In monkey and human ES cells, NANOG expression was restricted to undifferentiated cells. Furthermore, reactivation of the somatic cell-derived Nanog was tightly linked with nuclear reprogramming induced by cell hybridization with ES cells and by nuclear transplantation into enucleated oocytes. Notably, mouse Nanog (+/-) ES cells, which produced approximately half the amount of NANOG produced by wild-type ES cells, readily differentiated to multi-lineage cells in culture medium including LIF. The labile undifferentiated state was fully rescued by constitutive expression of exogenous Nanog. Thus, the activity of Nanog is tightly correlated with an undifferentiated state of cells even in nuclear reprogrammed somatic cells. Nanog may function as a key regulator for sustaining pluripotency in a dose-dependent manner.
Volume 122(1)
Pages 67-79
Published 2005-1
DOI 10.1016/j.mod.2004.08.008
PII S0925-4773(04)00213-8
PMID 15582778
MeSH Amino Acid Sequence Animals Blastocyst / metabolism* Cells, Cultured Chimera DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Embryo, Mammalian / metabolism Female Haplorhini Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism Humans Hybrid Cells Male Mice Mice, Inbred C57BL Molecular Sequence Data Morula / metabolism Nanog Homeobox Protein Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism* Pseudogenes Sequence Homology, Amino Acid
IF 1.9
Times Cited 173
Human embryonic stem cells KhES-1