Reference - Detail
| RRC ID | 2492 |
|---|---|
| Author | Blaser H, Reichman-Fried M, Castanon I, Dumstrei K, Marlow FL, Kawakami K, Solnica-Krezel L, Heisenberg CP, Raz E. |
| Title | Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow. |
| Journal | Dev Cell |
| Abstract |
The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration. |
| Volume | 11(5) |
| Pages | 613-27 |
| Published | 2006-11-1 |
| DOI | 10.1016/j.devcel.2006.09.023 |
| PII | S1534-5807(06)00452-7 |
| PMID | 17084355 |
| MeSH | Actins / physiology Animals Cell Membrane / physiology Cell Polarity Chemokine CXCL12 Chemokines, CXC / physiology Chemotaxis* Cytoplasm / physiology* Cytoskeleton / physiology Germ Cells / physiology* Myosins / physiology* Pseudopodia / physiology Receptors, CXCR4 / physiology Zebrafish / embryology Zebrafish / physiology* Zebrafish Proteins / physiology* |
| IF | 10.092 |
| Times Cited | 246 |
| WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Zebrafish | Reference |