| RRC ID |
2492
|
| 著者 |
Blaser H, Reichman-Fried M, Castanon I, Dumstrei K, Marlow FL, Kawakami K, Solnica-Krezel L, Heisenberg CP, Raz E.
|
| タイトル |
Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow.
|
| ジャーナル |
Dev Cell
|
| Abstract |
The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
|
| 巻・号 |
11(5)
|
| ページ |
613-27
|
| 公開日 |
2006-11-1
|
| DOI |
10.1016/j.devcel.2006.09.023
|
| PII |
S1534-5807(06)00452-7
|
| PMID |
17084355
|
| MeSH |
Actins / physiology
Animals
Cell Membrane / physiology
Cell Polarity
Chemokine CXCL12
Chemokines, CXC / physiology
Chemotaxis*
Cytoplasm / physiology*
Cytoskeleton / physiology
Germ Cells / physiology*
Myosins / physiology*
Pseudopodia / physiology
Receptors, CXCR4 / physiology
Zebrafish / embryology
Zebrafish / physiology*
Zebrafish Proteins / physiology*
|
| IF |
10.092
|
| 引用数 |
246
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ゼブラフィッシュ |
Reference |
