RRC ID 2507
Author Suchánková P, Kubaláková M, Kovárová P, Bartos J, Cíhalíková J, Molnár-Láng M, Endo TR, Dolezel J.
Title Dissection of the nuclear genome of barley by chromosome flow sorting.
Journal Theor Appl Genet
Abstract Isolation of mitotic chromosomes using flow cytometry is an attractive way to dissect nuclear genomes into their individual chromosomal components or portions of them. This approach is especially useful in plants with complex genomes, where it offers a targeted and hence economical approach to genome analysis and gene cloning. In several plant species, DNA of flow-sorted chromosomes has been used for isolation of molecular markers from specific genome regions, for physical mapping using polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH), for integration of genetic and physical maps and for construction of chromosome-specific DNA libraries, including those cloned in bacterial artificial chromosome vectors. Until now, chromosome analysis and sorting using flow cytometry (flow cytogenetics) has found little application in barley (2n = 14, 1C approximately 5,100 Mbp) because of the impossibility of discriminating and sorting individual chromosomes, except for the smallest chromosome 1H and some translocation chromosomes with DNA content significantly different from the remaining chromosomes. In this work, we demonstrate that wheat-barley ditelosomic addition lines can be used to sort any arm of barley chromosomes 2H-7H. Thus, the barley genome can be dissected into fractions representing only about 6-12% of the total genome. This advance makes the flow cytogenetics an attractive tool, which may greatly facilitate genome analysis and gene cloning in barley.
Volume 113(4)
Pages 651-9
Published 2006-8-1
DOI 10.1007/s00122-006-0329-8
PMID 16810504
MeSH Cell Nucleus / genetics Chromosomes, Plant* / ultrastructure Flow Cytometry / methods* Genome, Plant* Hordeum / genetics* Triticum / genetics
IF 4.439
Times Cited 37
Wheat LPGKU2088~LPGKU2104