| RRC ID |
2533
|
| Author |
Takahashi S, Onuma Y, Yokota C, Westmoreland JJ, Asashima M, Wright CV.
|
| Title |
Nodal-related gene Xnr5 is amplified in the Xenopus genome.
|
| Journal |
Genesis
|
| Abstract |
In Xenopus, six nodal-related genes (Xnrs) have been identified to date. We found numerous tandem duplications of Xnr5 in the Xenopus laevis and Xenopus tropicalis genomes that involve highly conserved copies of coding and regulatory regions. The duplicated versions of Xnr5 were expressed in both the superficial and deep layer of dorsal endoderm and in the deep layer of ventral endoderm, where the initial inducers of mesendoderm formation would be expected to be localized. Overexpression of secreted inhibitors of Xnrs led to a substantially enhanced transcription of the duplicated Xnr5 genes and Xnr6 in embryos. Therefore, Xnr5 and Xnr6 have a novel feedback loop to inhibit transcription of Xnr5 and Xnr6. These results suggest that the initialization of a strong Xnr5 and Xnr6 signal is enabled by the rapid transcription from multiple genes. The novel feedback loop may negatively regulate transcription of Xnr5s and Xnr6 to limit overproduction of these potent inducers, with the Xnr5/Xnr6 signal then activating positive (Xnrs) and negative (Xlefty) loops, which regulate the range of mesodermal tissues produced.
|
| Volume |
44(7)
|
| Pages |
309-21
|
| Published |
2006-7-1
|
| DOI |
10.1002/dvg.20217
|
| PMID |
16791846
|
| MeSH |
Animals
Base Sequence
DNA, Complementary / isolation & purification
Embryo, Nonmammalian
Gene Amplification*
Gene Expression Profiling
Gene Expression Regulation
Genetic Variation
Left-Right Determination Factors
Molecular Sequence Data
Nodal Signaling Ligands
Sequence Homology, Nucleic Acid
Transforming Growth Factor beta / metabolism
Xenopus Proteins / genetics*
Xenopus Proteins / isolation & purification
Xenopus laevis / genetics*
|
| IF |
1.76
|
| Times Cited |
18
|
|
WOS Category
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Clawed frogs / Newts |
|
