RRC ID 2618
著者 Yumoto I, Hirota K, Nodasaka Y, Yokota Y, Hoshino T, Nakajima K.
タイトル Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye.
ジャーナル Int J Syst Evol Microbiol
Abstract A psychrotolerant, obligately alkaliphilic bacterium, IDR2-2(T), which is able to reduce indigo dye was isolated from a fermented polygonum indigo (Polygonum tinctorium Lour.) produced in Date, Hokkaido, using a traditional Japanese method. The isolate grew at pH 9-12 but not at pH 7-8. It was a Gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. The isolate grew in 0-17 % (w/v) NaCl but not at NaCl concentrations higher than 18 % (w/v). Its major cellular fatty acids were C(14 : 0), C(16 : 0), C(16 : 1)9c and C(18 : 1)9c, and its DNA G+C content was 40.6 mol%. dl-lactic acid was the major end-product from d-glucose. No quinones could be detected. The peptidoglycan type was A4beta, Orn-d-Glu. A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain IDR2-2(T) is a member of the genus Alkalibacterium. DNA-DNA hybridization revealed low relatedness (less than 25 %) between the isolate and two phylogenetically related strains, Alkalibacterium olivapovliticus and Marinilactibacillus psychrotolerans. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Alkalibacterium psychrotolerans sp. nov. is proposed. The type strain is IDR2-2(T) (=JCM 12281(T)=NCIMB 13981(T)).
巻・号 54(Pt 6)
ページ 2379-2383
公開日 2004-11-1
DOI 10.1099/ijs.0.63130-0
PMID 15545487
MeSH Anaerobiosis Bacterial Typing Techniques Base Composition Cold Temperature DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / chemistry Fatty Acids / isolation & purification Fermentation Flagella Genes, rRNA Gentian Violet Glucose / metabolism Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / metabolism Hydrogen-Ion Concentration Indigo Carmine Indoles / metabolism* Japan Lactic Acid / metabolism Molecular Sequence Data Nucleic Acid Hybridization Oxidation-Reduction Peptidoglycan / chemistry Peptidoglycan / isolation & purification Phenazines Phylogeny Polygonum / microbiology* Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Sequence Analysis, DNA
IF 2.415
引用数 56
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12281?