RRC ID 2626
著者 Bhattacharjee RN, Park KS, Okada K, Kumagai Y, Uematsu S, Takeuchi O, Akira S, Iida T, Honda T.
タイトル Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus.
ジャーナル Biochem Biophys Res Commun
Abstract The thermostable direct hemolysin (TDH) is considered as a major virulence factor of Vibrio parahaemolyticus. We observed this potential in several human cancer cell lines by using the TDH-producing wild-type (RIMD2210633) as well as tdh-deletion mutant of V. parahaemolyticus and found that the deletion of tdh did not affect cytotoxicity to any of the cell lines tested. DNA fragmentation and annexin V staining showed that both wild-type and tdh-mutant trigger apoptosis in these cells. To understand the molecular basis of cell death in the absence of TDH, gene expression profile of human colon cancer cell line HCT116 infected with tdh-deletion mutant was carried out using human cDNA microarrays consisting of 33,000 known genes. In infected cells, differentially expressed genes including genes for early growth response, growth arrest and DNA damage, and activating transcription factor that affect programmed cell death pathways were detected. Interestingly, mutant strains having a deletion in type III secretion system 1 (TTSS1) failed to elicit DNA fragmentation in HCT116 cells. Our results strongly suggest that apoptosis requires functional TTSS1 and TTSS1-dependent translocation factor(s) to be associated with the host cell death, and thus pathogenesis of V. parahaemolyticus.
巻・号 335(2)
ページ 328-34
公開日 2005-9-23
DOI 10.1016/j.bbrc.2005.07.080
PII S0006-291X(05)01543-3
PMID 16061205
MeSH Annexin A5 / chemistry Apoptosis* Bacterial Toxins Blotting, Northern Blotting, Western Cell Line, Tumor DNA Damage DNA Fragmentation DNA, Complementary / metabolism Electrophoresis, Agar Gel Fluorescein-5-isothiocyanate / chemistry Gene Deletion Gene Expression Regulation, Neoplastic* Hemolysin Proteins / genetics* Hemolysin Proteins / metabolism Humans Immunoblotting Mutation Neoplasms / microbiology* Oligonucleotide Array Sequence Analysis / methods* Vibrio parahaemolyticus / genetics*
IF 2.985
引用数 16
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
病原微生物 RIMD 2210633