| RRC ID |
268
|
| 著者 |
Scholz S, Kurauchi K, Kinoshita M, Oshima Y, Ozato K, Schirmer K, Wakamatsu Y.
|
| タイトル |
Analysis of estrogenic effects by quantification of green fluorescent protein in juvenile fish of a transgenic medaka.
|
| ジャーナル |
Environ Toxicol Chem
|
| Abstract |
TheChgH-GFP strain of the teleost medaka contains a regulatory region of the estrogen-responsive choriogenin H (chgH) gene fussed to the green fluorescent protein (GFP) gene. The strain was developed for the identification of environmental estrogens by noninvasive analysis of GFP fluorescence. In the present study, a quantification method for GFP by image analysis was establishedand applied to the analysis of time- and concentration-dependent GFP fluorescence in juvenile fish. Concentration-response analyses were performed with fish exposed for 14 d to 17beta-estradiol (0.37-367 pM), genistein (0.37-367 nM), or p-nonylphenol (0.367-1,835 nM). By means of image analysis, it was shown that ChgH-GFP was induced at 183.5 pM or greater 17beta-estradiol. Time-course and recovery experiments indicated a strong accumulation of GFP in the liver. Results of reverse transcriptase-polymerase chain reaction analysis of choriogenin H and vitellogenin demonstrated induction of gene expression for the same rangeof concentrations as that for GFP analysis. Neither expression of these genes nor GFP fluorescence was induced by genistein and p-nonylphenol. Although the ChgH-GFP strain failed to detect these weakly estrogenic compounds, the simplicity of the GFP quantification during early life stages of fish offers promising possibilities for further developments of transgenic strains using different target regulatory sequences.
|
| 巻・号 |
24(10)
|
| ページ |
2553-61
|
| 公開日 |
2005-10-1
|
| DOI |
10.1897/04-525r.1
|
| PMID |
16268157
|
| MeSH |
Animals
Animals, Genetically Modified*
Antineoplastic Agents / toxicity*
Biomarkers / analysis
Dose-Response Relationship, Drug
Estradiol / toxicity*
Gene Expression Profiling
Genistein / toxicity*
Green Fluorescent Proteins / analysis*
Green Fluorescent Proteins / biosynthesis
Larva / growth & development
Larva / physiology
Liver / chemistry
Oryzias / genetics*
Oryzias / physiology*
Phenols / toxicity*
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution
Toxicity Tests / methods
Vitellogenins / biosynthesis
|
| IF |
3.152
|
| 引用数 |
17
|
|
WOS 分野
|
TOXICOLOGY
ENVIRONMENTAL SCIENCES
|
| リソース情報 |
| メダカ |
d-rR-Tg(ChgH-GFP) (TG132) |
