RRC ID 2728
Author Tong H, Dong X.
Title Lactobacillus concavus sp. nov., isolated from the walls of a distilled spirit fermenting cellar in China.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Two novel Lactobacillus strains, C-5-1(T) and HB5, were isolated from the walls of a distilled spirit fermenting cellar in Hebei province, China. The strains were Gram-positive, non-spore-forming, non-motile curved rods and were facultatively anaerobic. They produced 5% (-)-D/95% (+)-L lactic acid exclusively from glucose fermentation, but were catalase-negative. Both strains fermented N-acetylglucosamine, arbutin, salicin, cellobiose, maltose, trehalose and beta-gentiobiose. The mean DNA G+C content of the two strains was 46.9+/-0.8 mol% (46.4 mol% for the type strain, C-5-1(T)). Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain C-5-1(T) was clustered in the Lactobacillus casei/Pediococcus phylogenetic group and was closely related to Pediococcus dextrinicus JCM 5887(T) (97.9% similarity), followed by Lactobacillus algidus JCM 10491(T) (93.9% similarity). The DNA-DNA relatedness between the type strain C-5-1(T) and P. dextrinicus JCM 5887(T) and L. algidus JCM 10491(T) was only 5.4 and 4.3%, respectively. Therefore, based on phenotypic, genotypic and phylogenetic analyses, a novel Lactobacillus species, Lactobacillus concavus sp. nov., is proposed. The type strain is C-5-1(T) (=AS 1.5017(T)=LMG 22739(T)).
Volume 55(Pt 5)
Pages 2199-202
Published 2005-9
DOI 10.1099/ijs.0.63509-0
PII 55/5/2199
PMID 16166732
MeSH Base Composition China DNA, Bacterial / analysis DNA, Ribosomal / analysis Fermentation Genes, rRNA Genotype Geologic Sediments / microbiology* Lactic Acid / metabolism* Lactobacillus / classification Lactobacillus / genetics Lactobacillus / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Wine / microbiology*
IF 1.932
Times Cited 2
Pathogenic microorganisms JCM 5887? JCM 10491?