RRC ID 2766
Author Honda M, Nakagawa S, Hayashi K, Kitagawa N, Tsutsumi K, Nagata I, Niwa M.
Title Adrenomedullin improves the blood-brain barrier function through the expression of claudin-5.
Journal Cell. Mol. Neurobiol.
Abstract AIMS:Brain vascular endothelial cells secret Adrenomedullin (AM) has multifunctional biological properties. AM affects cerebral blood flow and blood-brain barrier (BBB) function. We studied the role of AM on the permeability and tight junction proteins of brain microvascular endothelial cells (BMEC).
METHODS:BMEC were isolated from rats and a BBB in vitro model was generated. The barrier functions were studied by measuring the transendothelial electrical resistance (TEER) and the permeability of sodium fluorescein and Evans' blue albumin. The expressions of tight junction proteins were analyzed using immunocytochemistry and immunoblotting.
RESULTS:AM increased TEER of BMEC monolayer dose-dependently. Immunocytochemistry revealed that AM enhanced the claudin-5 expression at a cell-cell contact site in a dose-dependent manner. Immunoblotting also showed an overexpression of claudin-5 in AM exposure.
CONCLUSIONS:AM therefore inhibits the paracellular transport in a BBB in vitro model through claudin-5 overexpression.
Volume 26(2)
Pages 109-18
Published 2006-3
DOI 10.1007/s10571-006-9028-x
PMID 16763778
MeSH Adrenomedullin / pharmacology Adrenomedullin / physiology* Animals Blood-Brain Barrier / cytology Blood-Brain Barrier / metabolism* Cells, Cultured Cerebrovascular Circulation Claudin-5 Electric Impedance Endothelial Cells / cytology Endothelial Cells / metabolism Endothelium, Vascular / cytology Male Membrane Proteins / metabolism* Permeability Rats Rats, Wistar Regional Blood Flow
IF 3.811
Times Cited 48