Reference - Detail
| RRC ID | 27720 |
|---|---|
| Author | Yabushita S, Fukamachi K, Kikuchi F, Ozaki M, Miyata K, Sukata T, Deguchi Y, Tanaka H, Kakehashi A, Kawamura S, Uwagawa S, Wanibuchi H, Suzui M, Alexander DB, Tsuda H. |
| Title | Twenty-one proteins up-regulated in human H-ras oncogene transgenic rat pancreas cancers are up-regulated in human pancreas cancer. |
| Journal | Pancreas |
| Abstract |
OBJECTIVES:We have established rat models of pancreatic ductal adenocarcinoma (PDAC) in which expression of a human H-ras(G12V) or K-ras(G12V) oncogene regulated by the Cre/lox system drives pancreatic carcinogenesis. Pancreatic ductal adenocarcinoma which develops in H-ras(G12V) and K-ras(G12V) transgenic rats is cytogenetically and histopathologically similar to human PDAC. The present study was designed to determine the feasibility of using the commercially available H-ras(G12V) transgenic rat to find diagnostic protein biomarkers for human pancreatic cancer. METHODS:For an animal model to be useful for searching for protein biomarkers for a disease, it is essential that proteins that are up-regulated in the model are also up-regulated in humans. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare H-ras(G12V) transgenic rat PDAC with surrounding normal pancreas tissue. RESULTS:We identified 30 up-regulated proteins in the H-ras(G12V) transgenic rat PDAC lesions; importantly, 21 human homologs of these 30 rat proteins are up-regulated in human pancreatic cancer patients. CONCLUSIONS:These results indicate that numerous proteins that are up-regulated in H-ras(G12V) transgenic rat PDAC are also up-regulated in human pancreatic cancer; therefore, this rat model can be used to search for diagnostic biomarkers for this disease. |
| Volume | 42(6) |
| Pages | 1034-9 |
| Published | 2013-8-1 |
| DOI | 10.1097/MPA.0b013e3182883624 |
| PMID | 23648844 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / metabolism* Adenocarcinoma / pathology Animals Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism Carcinoma, Pancreatic Ductal / genetics Carcinoma, Pancreatic Ductal / metabolism* Carcinoma, Pancreatic Ductal / pathology Chromatography, Liquid Disease Models, Animal Feasibility Studies Humans Mutation Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology Proteins / genetics Proteins / metabolism* Rats Rats, Transgenic Tandem Mass Spectrometry Up-Regulation ras Proteins / genetics ras Proteins / metabolism* |
| IF | 2.92 |
| Times Cited | 5 |
| WOS Category | GASTROENTEROLOGY & HEPATOLOGY |
| Resource | |
| Rats | SD-Tg(CAG-HRAS*G12V)250Htsu(strainID=790) |