| RRC ID |
27769
|
| Author |
Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
|
| Title |
Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
|
| Journal |
DNA Res
|
| Abstract |
To understand the epigenetic regulation required for germ cell-specific gene expression in the mouse, we analysed DNA methylation profiles of developing germ cells using a microarray-based assay adapted for a small number of cells. The analysis revealed differentially methylated sites between cell types tested. Here, we focused on a group of genomic sequences hypomethylated specifically in germline cells as candidate regions involved in the epigenetic regulation of germline gene expression. These hypomethylated sequences tend to be clustered, forming large (10 kb to ~9 Mb) genomic domains, particularly on the X chromosome of male germ cells. Most of these regions, designated here as large hypomethylated domains (LoDs), correspond to segmentally duplicated regions that contain gene families showing germ cell- or testis-specific expression, including cancer testis antigen genes. We found an inverse correlation between DNA methylation level and expression of genes in these domains. Most LoDs appear to be enriched with H3 lysine 9 dimethylation, usually regarded as a repressive histone modification, although some LoD genes can be expressed in male germ cells. It thus appears that such a unique epigenomic state associated with the LoDs may constitute a basis for the specific expression of genes contained in these genomic domains.
|
| Volume |
20(6)
|
| Pages |
549-65
|
| Published |
2013-12-1
|
| DOI |
10.1093/dnares/dst030
|
| PII |
dst030
|
| PMID |
23861320
|
| PMC |
PMC3859323
|
| MeSH |
Animals
Carcinogenesis / genetics
Cell Line
DNA Methylation*
Embryonic Stem Cells / metabolism
Epigenesis, Genetic
Female
Gene Duplication
Gene Expression Profiling
Germ Cells / metabolism*
Histones / metabolism
Male
Mice
Mice, Transgenic
Molecular Sequence Data
Neoplasm Proteins / genetics
Nuclear Proteins / genetics
Segmental Duplications, Genomic
Testis / metabolism
X Chromosome / genetics
X Chromosome / metabolism*
|
| IF |
4.009
|
| Times Cited |
9
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Mice |
RBRC00209
RBRC00821
RBRC00822 |
| Human and Animal Cells |
GS-DG1(AES0002) |
