| RRC ID |
278
|
| 著者 |
Kanda H, Igaki T, Kanuka H, Yagi T, Miura M.
|
| タイトル |
Wengen, a member of the Drosophila tumor necrosis factor receptor superfamily, is required for Eiger signaling.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
We identified Wengen, the first member of the Drosophila tumor necrosis factor receptor (TNFR) superfamily. Wengen is a type III membrane protein with conserved cysteine-rich residues (TNFR homology domain) in the extracellular domain, a hallmark of the TNFR superfamily. wengen mRNA is expressed at all stages of Drosophila development. The small-eye phenotype caused by an eye-specific overexpression of a Drosophila TNF superfamily ligand, Eiger, was dramatically suppressed by down-regulation of Wengen using RNA interference. In addition, Wengen and Eiger physically interacted with each other through their TNFR homology domain and TNF homology domain, respectively. These results suggest that Wengen can act as a component of a functional receptor for Eiger. Our identification of Wengen and further genetic analysis should provide increased understanding of the evolutionarily conserved roles of TNF/TNFR superfamily proteins in normal development, as well as in some pathophysiological conditions.
|
| 巻・号 |
277(32)
|
| ページ |
28372-5
|
| 公開日 |
2002-8-9
|
| DOI |
10.1074/jbc.C200324200
|
| PII |
S0021-9258(20)70226-7
|
| PMID |
12084706
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Genetically Modified
Blotting, Western
Cloning, Molecular
DNA, Complementary / metabolism
Down-Regulation
Drosophila / metabolism*
Drosophila Proteins / physiology*
Electrophoresis, Polyacrylamide Gel
Ligands
Membrane Proteins / physiology*
Microscopy, Fluorescence
Models, Genetic
Molecular Sequence Data
Phenotype
Precipitin Tests
Protein Structure, Tertiary
Receptors, Tumor Necrosis Factor / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Signal Transduction
Transfection
Tumor Necrosis Factor-alpha / metabolism*
|
| IF |
4.238
|
| 引用数 |
120
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
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