RRC ID 27920
Author Muñoz J, Cortés JC, Sipiczki M, Ramos M, Clemente-Ramos JA, Moreno MB, Martins IM, Pérez P, Ribas JC.
Title Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction.
Journal J. Cell Biol.
Abstract Cytokinesis has been extensively studied in different models, but the role of the extracellular cell wall is less understood. Here we studied this process in fission yeast. The essential protein Bgs4 synthesizes the main cell wall β(1,3)glucan. We show that Bgs4-derived β(1,3)glucan is required for correct and stable actomyosin ring positioning in the cell middle, before the start of septum formation and anchorage to the cell wall. Consequently, β(1,3)glucan loss generated ring sliding, oblique positioned rings and septa, misdirected septum synthesis indicative of relaxed rings, and uncoupling between a fast ring and membrane ingression and slow septum synthesis, suggesting that cytokinesis can progress with defective septum pushing and/or ring pulling forces. Moreover, Bgs4-derived β(1,3)glucan is essential for secondary septum formation and correct primary septum completion. Therefore, our results show that extracellular β(1,3)glucan is required for cytokinesis to connect the cell wall with the plasma membrane and for contractile ring function, as proposed for the equivalent extracellular matrix in animal cells.
Volume 203(2)
Pages 265-82
Published 2013-10-28
DOI 10.1083/jcb.201304132
PII jcb.201304132
PMID 24165938
PMC PMC3812973
MeSH Actomyosin / metabolism* Cell Membrane / metabolism* Cell Wall / metabolism* Cytokinesis* Genotype Glucosyltransferases / genetics Glucosyltransferases / metabolism Microbial Viability Microscopy, Fluorescence Phenotype Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Signal Transduction* Time Factors Time-Lapse Imaging Video Recording beta-Glucans / metabolism*
IF 8.784
Times Cited 23
WOS Category CELL BIOLOGY
Resource
Yeast FY7823