RRC ID 2801
著者 Urakubo T, Ogura A, Tominaga-Yoshino K.
タイトル Ultrastructural features of hippocampal CA1 synapses with respect to synaptic enhancement following repeated PKA activation.
ジャーナル Neurosci Lett
Abstract We reported previously that repeated activations, but not a single activation, of cyclic AMP-dependent protein kinase (PKA), led to a slowly developing (requiring approximately 1 week to develop) long-lasting (lasting > or = 3 weeks) enhancement of synaptic transmission efficiency in the organotypic slice culture of the rat hippocampus. It was accompanied by an increase in the number of synapses identified immunohistochemically. To answer the question of whether the "perforated synapse", which is known to occur transiently after the induction of long-term potentiation (LTP) in combination with the enlargement of postsynaptic density (PSD), is involved also in this slow/persistent synaptic enhancement, we examined the ultrastructural changes after the repeated activations of PKA. The answer was partially yes (occurrence of perforated synapses was increased) but partially no (the increase in the number of perforated synapses was not transient but persistent; mean apparent size of PSD did not increase). These results suggest that the mechanism of the slow/persistent synaptogenesis shares limited features with the mechanism of the quick/transient morphogenesis after LTP.
巻・号 407(1)
ページ 1-5
公開日 2006-10-16
DOI 10.1016/j.neulet.2006.07.057
PII S0304-3940(06)00766-X
PMID 16962236
MeSH Animals Animals, Newborn Colforsin / pharmacology Cyclic AMP-Dependent Protein Kinases / metabolism* Dendrites / ultrastructure Drug Administration Schedule Enzyme Activation / drug effects Excitatory Postsynaptic Potentials / drug effects Excitatory Postsynaptic Potentials / physiology Hippocampus / cytology* Long-Term Potentiation / drug effects Long-Term Potentiation / physiology Microscopy, Electron, Transmission / methods Organ Culture Techniques Rats Rats, Wistar Synapses / physiology* Synapses / ultrastructure* Synaptic Transmission / drug effects Synaptic Transmission / physiology* Time Factors
IF 2.274
引用数 9
WOS 分野 NEUROSCIENCES
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