| RRC ID |
28114
|
| Author |
Fujita M, Fujita Y, Iuchi S, Yamada K, Kobayashi Y, Urano K, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K.
|
| Title |
Natural variation in a polyamine transporter determines paraquat tolerance in Arabidopsis.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Polyamines (PAs) are ubiquitous, polycationic compounds that are essential for the growth and survival of all organisms. Although the PA-uptake system plays a key role in mammalian cancer and in plant survival, the underlying molecular mechanisms are not well understood. Here, we identified an Arabidopsis L-type amino acid transporter (LAT) family transporter, named RMV1 (resistant to methyl viologen 1), responsible for uptake of PA and its analog paraquat (PQ). The natural variation in PQ tolerance was determined in 22 Arabidopsis thaliana accessions based on the polymorphic variation of RMV1. An RMV1-GFP fusion protein localized to the plasma membrane in transformed cells. The Arabidopsis rmv1 mutant was highly resistant to PQ because of the reduction of PQ uptake activity. Uptake studies indicated that RMV1 mediates proton gradient-driven PQ transport. RMV1 overexpressing plants were hypersensitive to PA and PQ and showed elevated PA/PQ uptake activity, supporting the notion that PQ enters plant cells via a carrier system that inherently functions in PA transport. Furthermore, we demonstrated that polymorphic variation in RMV1 controls PA/PQ uptake activity. Our identification of a molecular entity for PA/PQ uptake and sensitivity provides an important clue for our understanding of the mechanism and biological significance of PA uptake.
|
| Volume |
109(16)
|
| Pages |
6343-7
|
| Published |
2012-4-17
|
| DOI |
10.1073/pnas.1121406109
|
| PII |
1121406109
|
| PMID |
22492932
|
| PMC |
PMC3341036
|
| MeSH |
Adaptation, Physiological / drug effects
Adaptation, Physiological / genetics
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Cation Transport Proteins / genetics*
Cation Transport Proteins / metabolism
Cell Membrane / metabolism
Chromosome Mapping
Chromosomes, Plant / genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Membrane Transport Proteins / genetics*
Membrane Transport Proteins / metabolism
Molecular Sequence Data
Mutation
Paraquat / metabolism*
Paraquat / pharmacology
Plants, Genetically Modified
Polyamines / metabolism*
Polyamines / pharmacology
Polymorphism, Single Nucleotide*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
| IF |
9.412
|
| Times Cited |
59
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pst11400
pst14522 |
