RRC ID 2814
Author Nakano T, Chen CL, Goto S, Lai CY, Hsu LW, Kawamoto S, Sasaki T, Lin YC, Kao YH, Ohmori N, Goto T, Sato S, Jawan B, Ono K, Cheng YF.
Title The immunological role of lipid transfer/metabolic proteins in liver transplantation tolerance.
Journal Transpl Immunol
Abstract BACKGROUND:In a rat tolerogenic orthotopic liver transplantation (OLT) model, recipient serum after OLT (post-OLT serum) has been reported to prevent allograft rejection. A previous proteomic study indicated that apolipoprotein E (apo-E), which is an important factor for cholesterol transportation, is expressed at the latter tolerogenic phase after OLT. It has also been known that adipose tissue-derived adipokine, adiponectin, is an essential factor for fatty acid catabolism. This study aimed to characterize the role of lipid transfer/metabolic proteins in liver transplantation tolerance.
METHODS:To identify the apo-E and adiponectin in post-OLT serum, Western analyses and enzyme-linked immunosorbent assay (ELISA) were performed, respectively. The immunosuppressive activities of those factors were evaluated by inhibition of the mixed lymphocyte reaction (MLR).
RESULTS:Western analyses showed that the mobility of apo-E was shifted at the latter tolerogenic phase after OLT in a natural tolerance model, and a similar phenomenon was confirmed in the serum of a drug-induced tolerance model (rejection model+cyclosporin A (CsA); 0 to 14 days) after cessation of CsA. Further study revealed that neutralization of modified apo-E in post-OLT serum reduced the immunosuppressive activity. Additionally, plasma adiponectin was significantly elevated at the latter phase after OLT, and possessed MLR-inhibitory activity.
CONCLUSIONS:These results suggest that the mobility shift of apo-E and/or the up-regulation of adiponectin may be necessary for overcoming the rejection, recovering the liver allograft function, and following tolerance induction in experimental OLT models, and may be useful as one indicator to surmise the prognosis after liver transplantation.
Volume 17(2)
Pages 130-6
Published 2007-2-1
DOI 10.1016/j.trim.2006.09.004
PII S0966-3274(06)00105-5
PMID 17306744
MeSH Adiponectin / blood* Adiponectin / metabolism Adiponectin / therapeutic use Animals Apolipoproteins E / blood* Apolipoproteins E / metabolism Cyclosporine / therapeutic use Glycosylation Graft Rejection / diagnosis* Graft Rejection / prevention & control Immunosuppression Immunosuppressive Agents / therapeutic use Lipid Metabolism Liver / chemistry Liver / metabolism Liver Transplantation / immunology* Male Models, Animal Prognosis Rats Rats, Inbred Strains Recombinant Proteins / therapeutic use Transplantation Tolerance*
IF 1.624
Times Cited 2
Rats PVG/Seac(strainID=113)