RRC ID 2834
Author Hano T, Oshima Y, Kinoshita M, Tanaka M, Mishima N, Ohyama T, Yanagawa T, Wakamatsu Y, Ozato K, Honjo T.
Title Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI Medaka (transgenic Oryzias latipes) exposed to ethinylestradiol.
Journal Environ Sci Technol
Abstract This study investigates the adverse and persistent effects of ethinylestradiol (EE2) on mature gonads of transgenic olvas-GFPIST II-YI medaka (Oryzias latipes). The measurement of gonadal size calculating the GFP-fluorescent area was used as a technique that enabled monitoring gonads in living specimens by GFP fluorescence. First, mature medaka were exposed to EE2 (47.8-522 ng/L) for 4 weeks. The gonads showed a significant reduction of the GFP-fluorescent area and Gonadosomatic Index in males exposed to EE2 at >216 ng/L and females exposed at 522 ng/L. Histologically, males at all treatments exhibited testis-ova and additionally, high connective tissue prevalence at > or =216 ng/L. Next, mature male medaka were exposed to EE2 (43.7-473 ng/L) for 3 weeks and allowed to depurate for 6 weeks, to investigate persistent effects of EE2. Continuous gonad observation showed that GFP began to decline 3 weeks after initial exposure to > or =215 ng/L. After depuration, the gonad's fluorescent areas gradually recovered, with no statistical difference at the end of the depuration period; normal spermatogenesis was present in these individuals. Alterations in GFP fluorescence clearly indicate the condition of the gonad in transgenic medaka and this strain showed a facilitated screening fish model to detect the adverse effects on the gonad by estrogenic chemicals.
Volume 41(4)
Pages 1473-9
Published 2007-2-15
DOI 10.1021/es0620134
PMID 17593759
MeSH Animals Animals, Genetically Modified Estrogens / pharmacology* Ethinyl Estradiol / pharmacology* Female Fluorescence Gonads / drug effects* Gonads / growth & development Gonads / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Male Oryzias / physiology*
IF 7.864
Times Cited 4
Medaka STII-YI-Tg(olvas-GFP) (TG137)