| RRC ID |
2834
|
| Author |
Hano T, Oshima Y, Kinoshita M, Tanaka M, Mishima N, Ohyama T, Yanagawa T, Wakamatsu Y, Ozato K, Honjo T.
|
| Title |
Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI Medaka (transgenic Oryzias latipes) exposed to ethinylestradiol.
|
| Journal |
Environ Sci Technol
|
| Abstract |
This study investigates the adverse and persistent effects of ethinylestradiol (EE2) on mature gonads of transgenic olvas-GFPIST II-YI medaka (Oryzias latipes). The measurement of gonadal size calculating the GFP-fluorescent area was used as a technique that enabled monitoring gonads in living specimens by GFP fluorescence. First, mature medaka were exposed to EE2 (47.8-522 ng/L) for 4 weeks. The gonads showed a significant reduction of the GFP-fluorescent area and Gonadosomatic Index in males exposed to EE2 at >216 ng/L and females exposed at 522 ng/L. Histologically, males at all treatments exhibited testis-ova and additionally, high connective tissue prevalence at > or =216 ng/L. Next, mature male medaka were exposed to EE2 (43.7-473 ng/L) for 3 weeks and allowed to depurate for 6 weeks, to investigate persistent effects of EE2. Continuous gonad observation showed that GFP began to decline 3 weeks after initial exposure to > or =215 ng/L. After depuration, the gonad's fluorescent areas gradually recovered, with no statistical difference at the end of the depuration period; normal spermatogenesis was present in these individuals. Alterations in GFP fluorescence clearly indicate the condition of the gonad in transgenic medaka and this strain showed a facilitated screening fish model to detect the adverse effects on the gonad by estrogenic chemicals.
|
| Volume |
41(4)
|
| Pages |
1473-9
|
| Published |
2007-2-15
|
| DOI |
10.1021/es0620134
|
| PMID |
17593759
|
| MeSH |
Animals
Animals, Genetically Modified
Estrogens / pharmacology*
Ethinyl Estradiol / pharmacology*
Female
Fluorescence
Gonads / drug effects*
Gonads / growth & development
Gonads / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
Male
Oryzias / physiology*
|
| IF |
7.864
|
| Times Cited |
4
|
|
WOS Category
|
ENVIRONMENTAL SCIENCES
ENGINEERING, ENVIRONMENTAL
|
| Resource |
| Medaka |
STII-YI-Tg(olvas-GFP) (TG137) |
