論文 - 詳細
| RRC ID | 2834 |
|---|---|
| 著者 | Hano T, Oshima Y, Kinoshita M, Tanaka M, Mishima N, Ohyama T, Yanagawa T, Wakamatsu Y, Ozato K, Honjo T. |
| タイトル | Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI Medaka (transgenic Oryzias latipes) exposed to ethinylestradiol. |
| ジャーナル | Environ Sci Technol |
| Abstract |
This study investigates the adverse and persistent effects of ethinylestradiol (EE2) on mature gonads of transgenic olvas-GFPIST II-YI medaka (Oryzias latipes). The measurement of gonadal size calculating the GFP-fluorescent area was used as a technique that enabled monitoring gonads in living specimens by GFP fluorescence. First, mature medaka were exposed to EE2 (47.8-522 ng/L) for 4 weeks. The gonads showed a significant reduction of the GFP-fluorescent area and Gonadosomatic Index in males exposed to EE2 at >216 ng/L and females exposed at 522 ng/L. Histologically, males at all treatments exhibited testis-ova and additionally, high connective tissue prevalence at > or =216 ng/L. Next, mature male medaka were exposed to EE2 (43.7-473 ng/L) for 3 weeks and allowed to depurate for 6 weeks, to investigate persistent effects of EE2. Continuous gonad observation showed that GFP began to decline 3 weeks after initial exposure to > or =215 ng/L. After depuration, the gonad's fluorescent areas gradually recovered, with no statistical difference at the end of the depuration period; normal spermatogenesis was present in these individuals. Alterations in GFP fluorescence clearly indicate the condition of the gonad in transgenic medaka and this strain showed a facilitated screening fish model to detect the adverse effects on the gonad by estrogenic chemicals. |
| 巻・号 | 41(4) |
| ページ | 1473-9 |
| 公開日 | 2007-2-15 |
| DOI | 10.1021/es0620134 |
| PMID | 17593759 |
| MeSH | Animals Animals, Genetically Modified Estrogens / pharmacology* Ethinyl Estradiol / pharmacology* Female Fluorescence Gonads / drug effects* Gonads / growth & development Gonads / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Male Oryzias / physiology* |
| IF | 7.864 |
| 引用数 | 4 |
| WOS 分野 | ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | STII-YI-Tg(olvas-GFP) (TG137) |