| RRC ID |
28452
|
| Author |
Niwa H, Toyooka Y, Shimosato D, Strumpf D, Takahashi K, Yagi R, Rossant J.
|
| Title |
Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation.
|
| Journal |
Cell
|
| Abstract |
Trophectoderm (TE), the first differentiated cell lineage of mammalian embryogenesis, forms the placenta, a structure unique to mammalian development. The differentiation of TE is a hallmark event in early mammalian development, but molecular mechanisms underlying this first differentiation event remain obscure. Embryonic stem (ES) cells can be induced to differentiate into the TE lineage by forced repression of the POU-family transcription factor, Oct3/4. We show here that this event can be mimicked by overexpression of Caudal-related homeobox 2 (Cdx2), which is sufficient to generate proper trophoblast stem (TS) cells. Cdx2 is dispensable for trophectoderm differentiation induced by Oct3/4 repression but essential for TS cell self-renewal. In preimplantation embryos, Cdx2 is initially coexpressed with Oct3/4 and they form a complex for the reciprocal repression of their target genes in ES cells. This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development.
|
| Volume |
123(5)
|
| Pages |
917-29
|
| Published |
2005-12-2
|
| DOI |
10.1016/j.cell.2005.08.040
|
| PII |
S0092-8674(05)00911-6
|
| PMID |
16325584
|
| MeSH |
Animals
Blastocyst* / cytology
Blastocyst* / physiology
CDX2 Transcription Factor
Cell Differentiation / physiology*
Cell Lineage*
Cells, Cultured
Enzyme Activation
Gene Expression Regulation, Developmental
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Mice
Mice, Inbred C57BL
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism*
Organic Cation Transport Proteins / genetics
Organic Cation Transport Proteins / metabolism*
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / physiology*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
38.637
|
| Times Cited |
750
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
pCdx2p-luc (RDB07098)
pPyCAG-EGFP-IZ (RDB08526)
pPyCAG-Cdx2ER-IP (RDB10265). |
