RRC ID 28613
著者 Robellet X, Fauque L, Legros P, Mollereau E, Janczarski S, Parrinello H, Desvignes JP, Thevenin M, Bernard P.
タイトル A genetic screen for functional partners of condensin in fission yeast.
ジャーナル G3 (Bethesda)
Abstract Mitotic chromosome condensation is a prerequisite for the accurate segregation of chromosomes during cell division, and the conserved condensin complex a central player of this process. However, how condensin binds chromatin and shapes mitotic chromosomes remain poorly understood. Recent genome-wide binding studies showing that in most species condensin is enriched near highly expressed genes suggest a conserved link between condensin occupancy and high transcription rates. To gain insight into the mechanisms of condensin binding and mitotic chromosome condensation, we searched for factors that collaborate with condensin through a synthetic lethal genetic screen in the fission yeast Schizosaccharomyces pombe. We isolated novel mutations affecting condensin, as well as mutations in four genes not previously implicated in mitotic chromosome condensation in fission yeast. These mutations cause chromosome segregation defects similar to those provoked by defects in condensation. We also identified a suppressor of the cut3-477 condensin mutation, which largely rescued chromosome segregation during anaphase. Remarkably, of the five genes identified in this study, four encode transcription co-factors. Our results therefore provide strong additional evidence for a functional connection between chromosome condensation and transcription.
巻・号 4(2)
ページ 373-81
公開日 2014-2-19
DOI 10.1534/g3.113.009621
PII g3.113.009621
PMID 24362309
PMC PMC3931570
MeSH Epistasis, Genetic* Mutation Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism
IF 2.781
引用数 8
WOS 分野 GENETICS & HEREDITY
リソース情報
酵母