論文 - 詳細
| RRC ID | 28636 |
|---|---|
| 著者 | Higashio H, Sato K, Nakano A. |
| タイトル | Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. |
| ジャーナル | Traffic |
| Abstract |
The coat protein complex II (COPII) is essential for vesicle formation from the endoplasmic reticulum (ER) and is composed of two heterodimeric subcomplexes, Sec23p/Sec24p and Sec13p/Sec31p, and the small guanosine triphosphatase Sar1p. In an effort to identify novel factors that may participate in COPII vesicle formation, we isolated SMY2, a yeast gene encoding a protein of unknown function, as a multicopy suppressor of the temperature-sensitive sec24-20 mutant. We found that even a low-copy expression of SMY2 was sufficient for the suppression of the sec24-20 phenotypes, and the chromosomal deletion of SMY2 led to a severe growth defect in the sec24-20 background. In addition, SMY2 exhibited genetic interactions with several other genes involved in the ER-to-Golgi transport. Subcellular fractionation analysis showed that Smy2p was a peripheral membrane protein fractionating together with COPII components. However, Smy2p was not loaded onto COPII vesicles generated in vitro. Interestingly, coimmunoprecipitation between Smy2p and the Sec23p/Sec24p subcomplex was specifically observed in sec23-1 and sec24-20 backgrounds, suggesting that this interaction was a prerequisite for the suppression of the sec24-20 phenotypes by overexpression of SMY2. We propose that Smy2p is located on the surface of the ER and facilitates COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. |
| 巻・号 | 9(1) |
| ページ | 79-93 |
| 公開日 | 2008-1-1 |
| DOI | 10.1111/j.1600-0854.2007.00668.x |
| PII | TRA668 |
| PMID | 17973654 |
| PMC | PMC2239301 |
| MeSH | COP-Coated Vesicles / metabolism* Chromosomes, Fungal Endoplasmic Reticulum / metabolism GTPase-Activating Proteins Genes, Fungal Golgi Apparatus / metabolism Immunoprecipitation Membrane Proteins / genetics Membrane Proteins / metabolism* Microscopy, Fluorescence Mutation Protein Transport Repressor Proteins / genetics Repressor Proteins / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Two-Hybrid System Techniques Vesicular Transport Proteins |
| IF | 4.045 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pYPL1 (RDB08718) pSMY1 (RDB08719) pSMY3 (RDB08720) pSMY2 (RDB08721) pSMY4 (RDB08722) pSMY5 (RDB08723) pSMY6 (RDB08724) pSMY7 (RDB08725) pSMY8 (RDB08726) pSMY9 (RDB08727) pSMY10 (RDB08728) pSMY11 (RDB08729) pSMY12 (RDB08730) pSMY13 (RDB08731) pJG-SEC23 (RDB08732) pJG-SEC24 (RDB08733) pEG-SEC24 (RDB08734) pEG-SMY2 (RDB08735) |