論文 - 詳細
| RRC ID | 28685 |
|---|---|
| 著者 | Yoshimura S, Yamamoto A, Misumi Y, Sohda M, Barr FA, Fujii G, Shakoori A, Ohno H, Mihara K, Nakamura N. |
| タイトル | Dynamics of Golgi matrix proteins after the blockage of ER to Golgi transport. |
| ジャーナル | J Biochem |
| Abstract |
When the ER to Golgi transport is blocked by a GTP-restricted mutant of Sar1p (H79G) in NRK-52E cells, most Golgi resident proteins are transported back into the ER. In contrast, the cis-Golgi matrix proteins GM130 and GRASP65 are retained in punctate cytoplasmic structures, namely Golgi remnants. Significant amounts of the medial-Golgi matrix proteins golgin-45, GRASP55 and giantin are retained in the Golgi remnants, but a fraction of these proteins relocates to the ER. Golgin-97, a candidate trans-Golgi network matrix protein, is retained in Golgi remnant-like structures, but mostly separated from GM130 and GRASP65. Interestingly, most Sec13p, a COPII component, congregates into larger cytoplasmic clusters soon after the microinjection of Sar1p(H79G), and these move to accumulate around the Golgi apparatus. Sec13p clusters remain associated with Golgi remnants after prolonged incubation. Electron microscopic analysis revealed that Golgi remnants are clusters of larger vesicles with smaller vesicles, many of which are coated. GM130 is mainly associated with larger vesicles and Sec13p with smaller coated vesicles. The Sec13p clusters disperse when p115 binding to the Golgi apparatus is inhibited. These results suggest that cis-Golgi matrix proteins resist retrograde transport flow and stay as true residents in Golgi remnants after the inhibition of ER to Golgi transport. |
| 巻・号 | 135(2) |
| ページ | 201-16 |
| 公開日 | 2004-2-1 |
| DOI | 10.1093/jb/mvh024 |
| PMID | 15047722 |
| MeSH | Autoantigens Biological Transport / physiology Cells, Cultured Cytoplasm / metabolism Endoplasmic Reticulum / metabolism* Golgi Apparatus / metabolism* Membrane Proteins / immunology Membrane Proteins / metabolism* Microinjections Microscopy, Electron Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism Mutation Nuclear Pore Complex Proteins Plasmids / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae Proteins / metabolism Vesicular Transport Proteins |
| IF | 2.476 |
| 引用数 | 43 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCAwt-EGFP-VSV-G(ts045) (RDB07990) |