RRC ID 28692
著者 Fang Y, Jaiseng W, Ma Y, Hu L, Yamazaki S, Zhang X, Hayafuji T, Shi L, Kuno T.
タイトル E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.
ジャーナル PLoS One
Abstract We previously identified three glycosylphosphatidylinositol (GPI)-anchored proteins including Ecm33, as multicopy suppressors of the phenotypes of a mutant allele of cis4(+) that encodes a zinc transporter in fission yeast. Here, we further identified two multicopy suppressor genes, ubi1 (+) and ubc4 (+), encoding ubiquitin-ribosomal fusion protein and ubiquitin conjugating enzyme E2, respectively. In addition, Ubi1 or Ubc4 overexpression failed to suppress the phenotypes of the double deletion of cis4 (+) and pub1 (+) gene, which encodes a HECT-type ubiquitin ligase E3. During exponential phase GFP-Ecm33 localized at the growing cell tips of the cell surface and the medial region in wild-type cells. Notably, during the post-exponential and stationary phase, GFP-Ecm33 in wild-type cells was internalized and mostly localized to the Golgi/endosomes, but it was still stably localized at the cell surface in Δpub1 cells. The Δpub1 cells showed osomoremedial phenotypes to various drugs indicating their defects in cell wall integrity. Altogether, our findings reveal a novel role for Pub1 in endocytosis of Ecm33 and regulation of cell wall integrity in fission yeast.
巻・号 9(1)
ページ e85238
公開日 2014-1-1
DOI 10.1371/journal.pone.0085238
PII PONE-D-13-11899
PMID 24454826
PMC PMC3891804
MeSH Calcineurin / metabolism Carbon-Nitrogen Ligases / metabolism* Cell Wall / metabolism Endocytosis* Genetic Pleiotropy / radiation effects* Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Response Elements / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription, Genetic Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism
IF 2.74
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY7683