論文 - 詳細
| RRC ID | 28743 |
|---|---|
| 著者 | Nakano A, Masuda K, Hiromoto T, Takahashi K, Matsumoto Y, Habib AG, Darwish AG, Yukawa M, Tsuchiya E, Ueno M. |
| タイトル | Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint. |
| ジャーナル | Mol Cell Biol |
| Abstract |
The spindle assembly checkpoint (SAC) monitors defects in kinetochore-microtubule attachment or lack of tension at kinetochores and arrests cells at prometaphase. In fission yeast, the double mutant between pot1Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene (rqh1-hd) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those intermediates. Here, we found that SAC-dependent prometaphase arrest occurred more frequently in pot1Δ rqh1-hd double mutants than in rqh1-hd single mutants. SAC-dependent prometaphase arrest also occurred more frequently in rqh1-hd single mutants after cells were released from DNA replication block compared to the rqh1-hd single mutant in the absence of exogenous insult to the DNA. In both cases, Mad2 foci persisted longer than usual at kinetochores, suggesting a defect in kinetochore-microtubule attachment. In pot1Δ rqh1-hd double mutants and rqh1-hd single mutants released from DNA replication block, SAC-dependent prometaphase arrest was suppressed by the removal of the recombination or replication intermediates. Our results indicate that the accumulation of recombination or replication intermediates induces SAC-dependent prometaphase arrest, possibly by affecting kinetochore-microtubule attachment. |
| 巻・号 | 34(8) |
| ページ | 1389-97 |
| 公開日 | 2014-4-1 |
| DOI | 10.1128/MCB.01704-13 |
| PII | MCB.01704-13 |
| PMID | 24469396 |
| PMC | PMC3993586 |
| MeSH | Animals Cell Cycle Proteins / genetics Chromosome Segregation / genetics Chromosomes, Fungal / genetics DNA Repair / genetics DNA, Ribosomal / genetics* Genes, cdc / genetics* Kinetochores / metabolism M Phase Cell Cycle Checkpoints / genetics Mitosis / genetics Mitosis / physiology Mutation / genetics Rad51 Recombinase / genetics* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Spindle Apparatus / genetics* Telomere / genetics* |
| IF | 3.611 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY18585 FY10134 FY18581 FY18537 |