論文 - 詳細
| RRC ID | 28848 |
|---|---|
| 著者 | Beumer KJ, Carroll D. |
| タイトル | Targeted genome engineering techniques in Drosophila. |
| ジャーナル | Methods |
| Abstract |
For a century, Drosophila has been a favored organism for genetic research. However, the array of materials and methods available to the Drosophila worker has expanded dramatically in the last decade. The most common gene targeting tools, zinc finger nucleases, TALENs, and RNA-guided CRISPR/Cas9, have all been adapted for use in Drosophila, both for simple mutagenesis and for gene editing via homologous recombination. For each tool, there exist a number of web sites, design applications, and delivery methods. The successful application of any of these tools also requires an understanding of methods for detecting successful genome modifications. This article provides an overview of the available gene targeting tools and their application in Drosophila. In lieu of simply providing a protocol for gene targeting, we direct the researcher to resources that will allow access to the latest research in this rapidly evolving field. |
| 巻・号 | 68(1) |
| ページ | 29-37 |
| 公開日 | 2014-6-15 |
| DOI | 10.1016/j.ymeth.2013.12.002 |
| PII | S1046-2023(13)00455-6 |
| PMID | 24412316 |
| PMC | PMC4048800 |
| MeSH | Animals Developmental Biology / methods* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Gene Expression Regulation, Developmental Gene Targeting / methods* Genetic Engineering / methods* Genome, Insect* Homologous Recombination Zinc Fingers / genetics |
| IF | 3.812 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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