RRC ID 28936
著者 Witke W, Schleicher M, Noegel AA.
タイトル Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins.
ジャーナル Cell
Abstract We generated by gene disruption Dictyostelium cells that lacked both the F-actin cross-linking proteins, alpha-actinin and gelation factor. Several major cell functions, such as growth, chemotaxis, phagocytosis, and pinocytosis, were apparently unaltered. However, in all double mutants, development was greatly impaired. After formation of aggregates, cells were very rarely able to form fruiting bodies. This ability was rescued when mutant and wild-type strains were mixed in a ratio of 70 to 30. The developmental program in the mutant was not arrested, since the expression pattern of early and late genes remained unchanged. Development of the mutant was rendered normal when a functional alpha-actinin gene was introduced and expressed, showing the morphogenetic defect to be due to the absence of the two F-actin cross-linking proteins. These findings suggest the existence of a functional network allowing mutual complementation of certain actin-binding proteins.
巻・号 68(1)
ページ 53-62
公開日 1992-1-10
DOI 10.1016/0092-8674(92)90205-q
PII 0092-8674(92)90205-Q
PMID 1732064
MeSH Actin Cytoskeleton / physiology* Actin Cytoskeleton / ultrastructure Actinin / genetics Actins / genetics* Actins / physiology Animals Carrier Proteins / genetics Cell Movement Chemotaxis Cytoskeleton / physiology Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / physiology* Genetic Vectors Microfilament Proteins / genetics Models, Biological Mutagenesis Phagocytosis Pinocytosis
IF 38.637
引用数 134
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 S00403 S00416 S90303