Reference - Detail
| RRC ID | 28993 |
|---|---|
| Author | Kim JY, Soede RD, Schaap P, Valkema R, Borleis JA, Van Haastert PJ, Devreotes PN, Hereld D. |
| Title | Phosphorylation of chemoattractant receptors is not essential for chemotaxis or termination of G-protein-mediated responses. |
| Journal | J Biol Chem |
| Abstract |
In several G-protein-coupled signaling systems, ligand-induced receptor phosphorylation by specific kinases is suggested to lead to desensitization via mechanisms including receptor/G-protein uncoupling, receptor internalization, and receptor down-regulation. We report here that elimination of phosphorylation of a chemoattractant receptor of Dictyostelium, either by site-directed substitution of the serines or by truncation of the C-terminal cytoplasmic domain, completely prevented agonist-induced loss of ligand binding but did not impair the adaptation of several receptor-mediated responses including the activation of adenylyl and guanylyl cyclases and actin polymerization. In addition, the phosphorylation-deficient receptors were capable of mediating chemotaxis, aggregation, and differentiation. We propose that for chemoattractant receptors agonist-induced phosphorylation regulates surface binding activity but other phosphorylation-independent mechanisms mediate response adaptation. |
| Volume | 272(43) |
| Pages | 27313-8 |
| Published | 1997-10-24 |
| DOI | 10.1074/jbc.272.43.27313 |
| PII | S0021-9258(18)68047-0 |
| PMID | 9341180 |
| MeSH | Actins / metabolism Adenylyl Cyclases / metabolism Amino Acid Substitution Animals Cell Aggregation Cell Differentiation Chemotaxis* Dictyostelium / physiology* Enzyme Activation GTP-Binding Proteins / metabolism* Guanylate Cyclase / metabolism Kinetics Mutagenesis, Site-Directed Phosphorylation Receptors, Cyclic AMP / biosynthesis Receptors, Cyclic AMP / physiology* Recombinant Proteins / biosynthesis Sequence Deletion Serine |
| IF | 4.238 |
| Times Cited | 75 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S00410 |