RRC ID 29025
Author Roelofs J, Snippe H, Kleineidam RG, Van Haastert PJ.
Title Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase.
Journal Biochem. J.
Abstract The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer of two cyclase domains arranged in an antiparallel fashion. Metazoan membrane-bound adenylate cyclases are composed of 12 transmembrane spanning regions, and two cyclase domains which function as a heterodimer and are activated by G-proteins. In contrast, membrane-bound guanylate cyclases have only one transmembrane spanning region and one cyclase domain, and are activated by extracellular ligands to form a homodimer. In the cellular slime mould, Dictyostelium discoideum, membrane-bound guanylate cyclase activity is induced after cAMP stimulation; a G-protein-coupled cAMP receptor and G-proteins are essential for this activation. We have cloned a Dictyostelium gene, DdGCA, encoding a protein with 12 transmembrane spanning regions and two cyclase domains. Sequence alignment demonstrates that the two cyclase domains are transposed, relative to these domains in adenylate cyclases. DdGCA expressed in Dictyostelium exhibits high guanylate cyclase activity and no detectable adenylate cyclase activity. Deletion of the gene indicates that DdGCA is not essential for chemotaxis or osmo-regulation. The knock-out strain still exhibits substantial guanylate cyclase activity, demonstrating that Dictyostelium contains at least one other guanylate cyclase.
Volume 354(Pt 3)
Pages 697-706
Published 2001-3-15
PMID 11237875
PMC PMC1221702
MeSH Adenylyl Cyclases / chemistry* Amino Acid Sequence Animals Cell Aggregation Cells, Cultured Cloning, Molecular Cyclic GMP / metabolism Dictyostelium / cytology Dictyostelium / enzymology* Dictyostelium / genetics Gene Deletion Guanylate Cyclase / chemistry* Guanylate Cyclase / genetics* Guanylate Cyclase / metabolism Guanylate Cyclase / physiology Membrane Proteins / chemistry* Membrane Proteins / genetics* Membrane Proteins / physiology Molecular Sequence Data Phylogeny Protein Conformation Protein Structure, Tertiary Protozoan Proteins* Sequence Homology, Amino Acid
IF 3.857
Times Cited 36
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Cellular slime molds S00337