Reference - Detail
| RRC ID | 29101 |
|---|---|
| Author | Nuñez-Corcuera B, Birch JL, Yamada Y, Williams JG. |
| Title | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| Journal | PLoS One |
| Abstract |
In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression. |
| Volume | 7(1) |
| Pages | e29895 |
| Published | 2012-1-1 |
| DOI | 10.1371/journal.pone.0029895 |
| PII | PONE-D-11-08613 |
| PMID | 22253818 |
| PMC | PMC3253789 |
| MeSH | Amino Acid Sequence Animals Base Sequence Basic-Leucine Zipper Transcription Factors / chemistry Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / isolation & purification Basic-Leucine Zipper Transcription Factors / metabolism* Binding Sites Cell Differentiation* / drug effects Cell Differentiation* / genetics Chromatography, Affinity Dictyostelium / cytology* Dictyostelium / drug effects Dictyostelium / genetics* Electrophoretic Mobility Shift Assay Gene Expression Regulation / drug effects Genes, Reporter / genetics Hexanones / pharmacology Hydrocarbons, Chlorinated / pharmacology Mass Spectrometry Models, Biological Molecular Sequence Data Mutation / genetics Promoter Regions, Genetic / genetics Protein Binding / drug effects Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / isolation & purification Protozoan Proteins / metabolism* Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / metabolism* Spores, Protozoan / cytology Spores, Protozoan / drug effects Spores, Protozoan / genetics Transcription, Genetic* / drug effects |
| IF | 2.74 |
| Times Cited | 3 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S90379 |