論文 - 詳細
| RRC ID | 29142 |
|---|---|
| 著者 | Tachibana M, Sugimoto K, Nozaki M, Ueda J, Ohta T, Ohki M, Fukuda M, Takeda N, Niida H, Kato H, Shinkai Y. |
| タイトル | G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. |
| ジャーナル | Genes Dev |
| Abstract |
Covalent modification of histone tails is crucial for transcriptional regulation, mitotic chromosomal condensation, and heterochromatin formation. Histone H3 lysine 9 (H3-K9) methylation catalyzed by the Suv39h family proteins is essential for establishing the architecture of pericentric heterochromatin. We recently identified a mammalian histone methyltransferase (HMTase), G9a, which has strong HMTase activity towards H3-K9 in vitro. To investigate the in vivo functions of G9a, we generated G9a-deficient mice and embryonic stem (ES) cells. We found that H3-K9 methylation was drastically decreased in G9a-deficient embryos, which displayed severe growth retardation and early lethality. G9a-deficient ES cells also exhibited reduced H3-K9 methylation compared to wild-type cells, indicating that G9a is a dominant H3-K9 HMTase in vivo. Importantly, the loss of G9a abolished methylated H3-K9 mostly in euchromatic regions. Finally, G9a exerted a transcriptionally suppressive function that depended on its HMTase activity. Our results indicate that euchromatic H3-K9 methylation regulated by G9a is essential for early embryogenesis and is involved in the transcriptional repression of developmental genes. |
| 巻・号 | 16(14) |
| ページ | 1779-91 |
| 公開日 | 2002-7-15 |
| DOI | 10.1101/gad.989402 |
| PMID | 12130538 |
| PMC | PMC186403 |
| MeSH | Acetylation Animals Embryonic and Fetal Development Euchromatin / metabolism* Gene Targeting Germ Cells Histone Methyltransferases Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism* Histones / metabolism* Lysine / metabolism* Methylation Methyltransferases / genetics Methyltransferases / metabolism* Mice Mice, Inbred C57BL Mice, Knockout Protein Methyltransferases Repressor Proteins / genetics Repressor Proteins / metabolism* Transcription, Genetic |
| IF | 9.527 |
| 引用数 | 795 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pZErO-2.1 mG9a-S (RDB05727) pZErO-2.1 mG9a-L (RDB05728) |