RRC ID 29150
Author Sato K, Sato M, Nakano A.
Title Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer.
Journal J. Cell Biol.
Abstract Rer1p, a yeast Golgi membrane protein, is required for the retrieval of a set of endoplasmic reticulum (ER) membrane proteins. We present the first evidence that Rer1p directly interacts with the transmembrane domain (TMD) of Sec12p which contains a retrieval signal. A green fluorescent protein (GFP) fusion of Rer1p rapidly cycles between the Golgi and the ER. Either a lesion of coatomer or deletion of the COOH-terminal tail of Rer1p causes its mislocalization to the vacuole. The COOH-terminal Rer1p tail interacts in vitro with a coatomer complex containing alpha and gamma subunits. These findings not only give the proof that Rer1p is a novel type of retrieval receptor recognizing the TMD in the Golgi but also indicate that coatomer actively regulates the function and localization of Rer1p.
Volume 152(5)
Pages 935-44
Published 2001-3-5
PMID 11238450
PMC PMC2198819
MeSH Amino Acid Motifs Amino Acid Sequence Coat Protein Complex I / chemistry Coat Protein Complex I / genetics Coat Protein Complex I / metabolism* Endoplasmic Reticulum / metabolism* Fungal Proteins / chemistry Fungal Proteins / genetics Fungal Proteins / metabolism* Golgi Apparatus / chemistry* Golgi Apparatus / metabolism Guanine Nucleotide Exchange Factors Intracellular Membranes / chemistry Intracellular Membranes / metabolism Lysine / genetics Lysine / metabolism Membrane Glycoproteins / chemistry Membrane Glycoproteins / metabolism* Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Models, Biological Molecular Sequence Data Mutation Protein Binding Protein Structure, Tertiary Protein Subunits Protein Transport Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins* Sequence Alignment Vacuoles / metabolism Vesicular Transport Proteins
IF 8.891
Times Cited 108
DNA material pSKY5-RER1-0 (RDB08615) pSKY5-RER1-5 (RDB08616) pSKY5-RER1-10 (RDB08617) pSKY5-RER1-15 (RDB08618) pSKY5-RER1-20 (RDB08619) pSKY5-RER1-25 (RDB08620) pSKY5-RER1 G179A (RDB08621) pSKY5-RER1-K180S (RDB08622) pSKY5-RER1-K181S (RDB08623) pSKY5-RER1-K182S (RDB08624) pSKY5-RER1-Y183A (RDB08625) pSKY5-RER1-Y173A (RDB08626) pSKY5-RER1-KKSS (RDB08627) pSKY5 (RDB08655) 316-HA-Ste2 (RDB08791) HA-te2-Rer1p chimera (RDB08792) GST-Wbp1 (RDB08793) GST-Wbp1-SS (RDB08794) GST-Rer1C28 (RDB08795) GST-Rer1C28-KKSS (RDB08796) GST-Rer1C28-Y173A (RDB08797) GST-Rer1C28-Y183A (RDB08798) Pgap-Dap2 (RDB08799) Pgap-DSD (RDB08800) Pgap-DSD-NQ/L (RDB08801) Pgap-DSD-SY/L (RDB08802) Pgap-DSD-+2L (RDB08803) Pgap-DSD-L19 (RDB08804)