RRC ID 29174
著者 Sato M, Sato K, Nakano A.
タイトル Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain.
ジャーナル J Cell Biol
Abstract Yeast Sec12p is a type II transmembrane protein in the ER, which is essential for the formation of transport vesicles. From biochemical and morphological lines of evidence, we have proposed that Sec12p is localized to the ER by two mechanisms: static retention in the ER and dynamic retrieval from the early Golgi compartment. We have also shown that Rer1p, a membrane protein in the Golgi, is required for correct localization of Sec12p. In the present study, we have performed a systematic analysis to determine the ER localization signals in Sec12p corresponding to these two mechanisms. Both the transmembrane domain (TMD) and the NH2-terminal cytoplasmic domain of Sec12p show the ability to localize the protein to the ER. The effect of the TMD is potent and sufficient by itself for the ER localization and is strongly dependent on Rer1p. On the other hand, the cytoplasmic domain shows a moderate ER-localization capability which is independent of Rer1p. The rate of mannosyl modification has been measured to distinguish between retention and retrieval. The cytoplasmic domain significantly delays the transport from the ER to the cis-Golgi. In contrast, the TMD shows only a subtle retardation in the transport from the ER to the cis-Golgi but strictly prevents the transport beyond there. From these observations, we conclude that the TMD mainly acts as the retrieval signal and the cytoplasmic domain contains the retention signal. This study not only supports the two-mechanisms hypothesis but also provides powerful tools to dissect the two.
巻・号 134(2)
ページ 279-93
公開日 1996-7-1
DOI 10.1083/jcb.134.2.279
PMID 8707815
PMC PMC2120888
MeSH Amino Acid Sequence Base Sequence Binding Sites Cloning, Molecular Cytoplasm / metabolism DNA, Fungal Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / genetics Endoplasmic Reticulum / metabolism* Fungal Proteins / genetics Fungal Proteins / metabolism* Guanine Nucleotide Exchange Factors Mannose Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Membrane Proteins / metabolism* Molecular Sequence Data Mutation Protein Sorting Signals / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins* Vesicular Transport Proteins
IF 8.811
引用数 115
WOS 分野 CELL BIOLOGY
リソース情報
遺伝子材料 pMS2-3 (pSSS-1) (RDB08842) pDDD-2 (pSD1-2 pMD2-6) (RDB08843) pDSS-2 (pSD4-2) (RDB08844) pDDS-2 (pSD3-2) (RDB08845) pSDS-2 (pSD2-2) (RDB08846) pSSD-2 (pSD7-2) (RDB08847) pDSD-2 (pSD5-2) (RDB08848) pSDD-2 (pSD6-2) (RDB08849) pDSDm F-A (RDB08850) pDSDm SY-A (RDB08851) DSDm N-A (RDB08852) DSDm Q-A (RDB08853) DSDm NQ-A (RDB08854) DSDm A10 (RDB08855) DSDm N-L (RDB08856) DSDm Q-L (RDB08857) DSDm NQ-L (RDB08858) L7 (pDSDm-L7) (RDB08859) LeuX19 (RDB08860) pD4Dm-2 (RDB08861) pD20Dm-2 (RDB08862) p4DDm-2 (RDB08863)