RRC ID 29219
著者 Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y.
タイトル Inhibitory effect of p53 on mitochondrial content and function during adipogenesis.
ジャーナル Biochem Biophys Res Commun
Abstract The p53 protein is known as a guardian of the genome and is involved in energy metabolism. Since the metabolic system is uniquely regulated in each tissue, we have anticipated that p53 also would play differential roles in each tissue. In this study, we focused on the functions of p53 in white adipose tissue (adipocytes) and skeletal muscle (myotubes), which are important peripheral tissues involved in energy metabolism. We found that in 3T3-L1 preadipocytes, but not in C2C12 myoblasts, p53 stabilization or overexpression downregulates the expression of Ppargc1a, a master regulator of mitochondrial biogenesis. Next, by using p53-knockdown C2C12 myotubes or 3T3-L1 preadipocytes, we further examined the relationship between p53 and mitochondrial regulation. In C2C12 myoblasts, p53 knockdown did not significantly affect Ppargc1a expression and mtDNA, but did suppress differentiation to myotubes, as previously reported. However, in 3T3-L1 preadipocytes and mouse embryonic fibroblasts, p53 downregulation enhanced both differentiation into adipocytes and mitochondrial DNA content. Furthermore, p53-depleted 3T3-L1 cells showed increase in mitochondrial proteins and enhancement of both Citrate Synthase and Complex IV activities during adipogenesis. These results show that p53 differentially regulates cell differentiation and mitochondrial biogenesis between adipocytes and myotubes, and provide evidence that p53 is an inhibitory factor of mitochondrial regulation in adipocyte lineage.
巻・号 446(1)
ページ 91-7
公開日 2014-3-28
DOI 10.1016/j.bbrc.2014.02.059
PII S0006-291X(14)00321-0
PMID 24565844
MeSH 3T3-L1 Cells Adipocytes / cytology Adipocytes / metabolism Adipogenesis / genetics Adipogenesis / physiology* Animals Cell Differentiation / genetics Cell Differentiation / physiology Cell Line Cell Lineage / genetics Cell Lineage / physiology Cells, Cultured DNA, Mitochondrial / genetics DNA, Mitochondrial / metabolism Fibroblasts / cytology Fibroblasts / metabolism Gene Expression Gene Knockdown Techniques Genes, p53 Mice Mice, Knockout Mitochondria / genetics Mitochondria / metabolism* Myoblasts, Skeletal / cytology Myoblasts, Skeletal / metabolism Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Transcription Factors / genetics Tumor Suppressor Protein p53 / antagonists & inhibitors Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism*
IF 2.985
引用数 9
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
実験動物マウス RBRC01361