RRC ID 29500
Author Fukunishi K, Miyakubi K, Hatanaka M, Otsuru N, Hirata A, Shimoda C, Nakamura T.
Title The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3.
Journal Mol Biol Cell
Abstract The spore is a dormant cell that is resistant to various environmental stresses. As compared with the vegetative cell wall, the spore wall has a more extensive structure that confers resistance on spores. In the fission yeast Schizosaccharomyces pombe, the polysaccharides glucan and chitosan are major components of the spore wall; however, the structure of the spore surface remains unknown. We identify the spore coat protein Isp3/Meu4. The isp3 disruptant is viable and executes meiotic nuclear divisions as efficiently as the wild type, but isp3∆ spores show decreased tolerance to heat, digestive enzymes, and ethanol. Electron microscopy shows that an electron-dense layer is formed at the outermost region of the wild-type spore wall. This layer is not observed in isp3∆ spores. Furthermore, Isp3 is abundantly detected in this layer by immunoelectron microscopy. Thus Isp3 constitutes the spore coat, thereby conferring resistance to various environmental stresses.
Volume 25(10)
Pages 1549-59
Published 2014-5-1
DOI 10.1091/mbc.E13-12-0731
PII mbc.E13-12-0731
PMID 24623719
PMC PMC4019487
MeSH Amidohydrolases Amino Acid Sequence Cell Wall / chemistry Cell Wall / enzymology Cell Wall / genetics* Chitin / biosynthesis Chitin Synthase / genetics* Chitosan / metabolism Ethanol / toxicity Green Fluorescent Proteins / genetics Hot Temperature / adverse effects Meiosis / genetics Microscopy, Immunoelectron Molecular Chaperones / biosynthesis Molecular Chaperones / genetics* Phosphoproteins / biosynthesis Phosphoproteins / genetics* Proteolysis Schizosaccharomyces / cytology* Schizosaccharomyces pombe Proteins / biosynthesis Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Spores, Fungal / cytology* Stress, Physiological
IF 3.791
Times Cited 5
WOS Category CELL BIOLOGY
Resource
Yeast FY7520 FY17852 FY17853 FY17854 FY17858 FY17862 FY17873 FY17875 FY14016 FY17731 FY7456 FY7816 FY7051 BY19600