RRC ID 29812
Author Farr SB, Touati D, Kogoma T.
Title Effects of oxygen stress on membrane functions in Escherichia coli: role of HPI catalase.
Journal J Bacteriol
Abstract Different conditions of oxidative stress were used to study their effects on membrane transport in Escherichia coli K-12. The oxidizing conditions included H2O2, plumbagin (a redox cycling compound that generates superoxide radicals [O2-]), and increased partial pressure of oxygen. Both superoxide radical-generating conditions and H2O2 treatments were found to cause a rapid decrease in proton motive force-dependent and -independent transport. H2O2-pretreated cells had the ability to rapidly recover both proton motive force-dependent and -independent transport. The induction required transcription and translation and was dependent on oxyR+ and katG+, providing evidence that these genes play crucial roles in the rapid recovery of transport. The effects of oxidatively induced loss of proton motive force on cell growth and macromolecular synthesis were also investigated.
Volume 170(4)
Pages 1837-42
Published 1988-4-1
DOI 10.1128/jb.170.4.1837-1842.1988
PMID 2832383
PMC PMC211039
MeSH Biological Transport, Active Catalase / genetics Catalase / metabolism* Cell Membrane / metabolism Chloramphenicol / pharmacology Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli / ultrastructure Genes, Bacterial Genes, Regulator Hydrogen Peroxide / pharmacology Mutation Naphthoquinones / pharmacology Oxidation-Reduction Oxygen / metabolism* Oxygen / pharmacology Protein Biosynthesis Protons Rifampin / pharmacology Transcription, Genetic
IF 3.006
Times Cited 75
Prokaryotes E. coli ME9340