RRC ID |
29882
|
著者 |
Mount DW, Low KB, Edmiston SJ.
|
タイトル |
Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations.
|
ジャーナル |
J Bacteriol
|
Abstract |
Three mutations, denoted lex-1, -2 and -3, which increase the sensitivity of Escherichia coli K-12 to ultraviolet light (UV) and ionizing radiation, have been found by three-factor transduction crosses to be closely linked to uvrA on the E. coli K-12 linkage map. Strains bearing these mutations do not appear to be defective in genetic recombination although in some conjugational crosses they may fail to produce a normal yield of genetic recombinants depending upon the time of mating and the marker selected. The mutagenic activity of UV is decreased in the mutant strains. After irradiation with UV, cultures of the strains degrade their deoxyribonucleic acid at a high rate, similar to recA(-) mutant strains. Stable lex(+)/lec(-) heterozygotes are found to have the mutant radiation-sensitive phenotype of haploid lex(-) strains.
|
巻・号 |
112(2)
|
ページ |
886-93
|
公開日 |
1972-11-1
|
DOI |
10.1128/jb.112.2.886-893.1972
|
PMID |
4343824
|
PMC |
PMC251500
|
MeSH |
Alkanesulfonates / pharmacology
Cell Survival
Chromosome Mapping
Chromosomes, Bacterial
Conjugation, Genetic
Crosses, Genetic
DNA, Bacterial / radiation effects
Drug Resistance, Microbial
Escherichia coli / drug effects
Escherichia coli / radiation effects*
Genes, Dominant
Genetic Linkage
Mutagens
Mutation*
Nitrosoguanidines
Nucleic Acid Denaturation
Phenotype
Radiation Effects*
Recombination, Genetic
Transduction, Genetic
Ultraviolet Rays*
|
IF |
3.006
|
リソース情報 |
原核生物(大腸菌) |
ME8414 |