RRC ID 29882
Author Mount DW, Low KB, Edmiston SJ.
Title Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations.
Journal J Bacteriol
Abstract Three mutations, denoted lex-1, -2 and -3, which increase the sensitivity of Escherichia coli K-12 to ultraviolet light (UV) and ionizing radiation, have been found by three-factor transduction crosses to be closely linked to uvrA on the E. coli K-12 linkage map. Strains bearing these mutations do not appear to be defective in genetic recombination although in some conjugational crosses they may fail to produce a normal yield of genetic recombinants depending upon the time of mating and the marker selected. The mutagenic activity of UV is decreased in the mutant strains. After irradiation with UV, cultures of the strains degrade their deoxyribonucleic acid at a high rate, similar to recA(-) mutant strains. Stable lex(+)/lec(-) heterozygotes are found to have the mutant radiation-sensitive phenotype of haploid lex(-) strains.
Volume 112(2)
Pages 886-93
Published 1972-11
DOI 10.1128/JB.112.2.886-893.1972
PMID 4343824
PMC PMC251500
MeSH Alkanesulfonates / pharmacology Cell Survival Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Crosses, Genetic DNA, Bacterial / radiation effects Drug Resistance, Microbial Escherichia coli / drug effects Escherichia coli / radiation effects* Genes, Dominant Genetic Linkage Mutagens Mutation* Nitrosoguanidines Nucleic Acid Denaturation Phenotype Radiation Effects* Recombination, Genetic Transduction, Genetic Ultraviolet Rays*
IF 3.234
Prokaryotes E. coli ME8414