RRC ID 29927
著者 Lloyd RG, Low B, Godson GN, Birge EA.
タイトル Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
ジャーナル J Bacteriol
Abstract A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.
巻・号 120(1)
ページ 407-15
公開日 1974-10-1
DOI 10.1128/jb.120.1.407-415.1974
PMID 4607624
PMC PMC245777
MeSH Alleles Cell Survival Chromosome Mapping Conjugation, Genetic DNA Nucleotidyltransferases / biosynthesis DNA, Bacterial / biosynthesis Escherichia coli* / enzymology Escherichia coli* / growth & development Escherichia coli* / isolation & purification Escherichia coli* / metabolism Escherichia coli* / radiation effects Genes Genetic Complementation Test Genetic Linkage Mutagens Mutation* Nitrosoguanidines Phenotype Radiation Effects Recombination, Genetic* Temperature Thymidine / metabolism Transduction, Genetic Tritium Ultraviolet Rays
IF 3.006
リソース情報
原核生物(大腸菌) ME5401 ME7101