RRC ID 30025
Author Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N.
Title New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
Journal Gene
Abstract We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.
Volume 32(3)
Pages 369-79
Published 1984-12-1
DOI 10.1016/0378-1119(84)90012-x
PII 0378-1119(84)90012-X
PMID 6099322
MeSH DNA Transposable Elements* Escherichia coli / genetics* Genetic Engineering / methods* Genetic Vectors Lac Operon* Mutation* Plasmids beta-Galactosidase / genetics
IF 2.638
Times Cited 593
Prokaryotes E. coli ME9018