| RRC ID |
30025
|
| Author |
Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N.
|
| Title |
New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
|
| Journal |
Gene
|
| Abstract |
We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.
|
| Volume |
32(3)
|
| Pages |
369-79
|
| Published |
1984-12-1
|
| DOI |
10.1016/0378-1119(84)90012-x
|
| PII |
0378-1119(84)90012-X
|
| PMID |
6099322
|
| MeSH |
DNA Transposable Elements*
Escherichia coli / genetics*
Genetic Engineering / methods*
Genetic Vectors
Lac Operon*
Mutation*
Plasmids
beta-Galactosidase / genetics
|
| IF |
2.984
|
| Times Cited |
593
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Prokaryotes E. coli |
ME9018 |
