Reference - Detail
| RRC ID | 30025 |
|---|---|
| Author | Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N. |
| Title | New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. |
| Journal | Gene |
| Abstract |
We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described. |
| Volume | 32(3) |
| Pages | 369-79 |
| Published | 1984-12-1 |
| DOI | 10.1016/0378-1119(84)90012-x |
| PII | 0378-1119(84)90012-X |
| PMID | 6099322 |
| MeSH | DNA Transposable Elements* Escherichia coli / genetics* Genetic Engineering / methods* Genetic Vectors Lac Operon* Mutation* Plasmids beta-Galactosidase / genetics |
| IF | 2.984 |
| Times Cited | 593 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 20 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME9018 |